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Table 1 Proteins enriched in GST-CPE116–319 fraction as detected by mass spectrometry

From: A strategy for enrichment of claudins based on their affinity to Clostridium perfringens enterotoxin

AN

Protein

MWapp

MW

SC

N

R

comment

Q63400

Claudin-3

20000

23269

298

6

11.9

tight junction

O35054

Claudin-4

20000

22150

170

3

11.0

tight junction

Q9Z1L1

Claudin-7

20000

22416

157

2

11.0

tight junction

P56745

Claudin-1

20000

22850

171

3

10.2

tight junction

Q8CFI7

DNA-directed RNA polymerase II subunit RPB2

120000

133911

1911

42

7.7

nuclear

P60898

DNA-directed RNA polymerase II subunit RPB9

10000

14532

234

3

7.7

nuclear

Q9ERU9

Ran-binding protein 2#

130000

341091

579

14

7.6

nuclear

P97760

DNA-directed RNA polymerase II subunit RPB3

30000

31311

470

7

6.2

nuclear, cytosolic

Q99M87

DnaJ homolog subfamily A member 3

36000

52443

90

2

6.1

mitochondrial

O15514

DNA-directed RNA polymerase II subunit RPB4

14000

16311

134

2

6.0

nuclear

P62832

60S ribosomal protein L23

14000

14865

296

7

5.5

ribosomal

Q9R112

Sulfide:quinone oxidoreductase

38000

50340

916

18

4.9

mitochondrial

Q5RK28

Normal mucosa of esophagus-specific gene 1 protein

5000

9598

143

3

4.2

nuclear

Q9D6M3

Mitochondrial glutamate carrier 1

27000

34670

153

4

4.1

mitochondrial

Q5I0H3

Small ubiquitin-related modifier 1 (SUMO-1)

85000

11557

147

3

4.1

nuclear, cytosolic

P46061

Ran GTPase-activating protein 1

85000

63616

506

9

4.0

nuclear, cytosolic

P29147

D-beta-hydroxybutyrate dehydrogenase

25000

38202

129

3

3.9

mitochondrial

Q62425

NADH dehydrogenase 1 alpha subcomplex

7000

9327

234

5

3.3

mitochondrial

P13437

3-ketoacyl-CoA thiolase

40000

41871

589

14

3.3

mitochondrial

Q9JIA7

Sphingosine kinase 2

64000

65618

109

2

3.3

cytosolic

Q8BH59

Calcium-binding mitochondrial carrier protein Aralar1

62000

74570

394

8

3.2

mitochondrial

Q66X93

Staphylococcal nuclease domain-containing protein 1

99000

101952

795

18

3.1

nuclear

Q8N163

KIAA 1967

110000

102902

613

12

3.1

nuclear

Q5I0E6

RNA polymerase II-associated protein 2

64000

67892

140

3

3.1

not assigned1

Q925I1

ATPase family AAA domain-containing protein 3

53000

66742

565

12

3.0

mitochondrial

  1. Each protein was identified in at least two of three independent experiments (including one experiment applying inverse labeling). AN, UniProtKB/SwissProt protein accession number; MWapp, apparent molecular weight [Da] according to position in gel; MW, molecular weight [Da] according to UniProtKB/SwissProt; SC, protein (Mascot™) score (highest value of individual experiment); N, highest number of peptides identified in individual experiment; R, ratio between MS peak intensities of peptides originating from GST-CPE116–319 and GST elution fractions; comment, cellular component according to UniProtKB/SwissProt (1nuclear/cytosolic according to [22]); #, protein fragment