Skip to main content

Advertisement

Table 1 Proteins enriched in GST-CPE116–319 fraction as detected by mass spectrometry

From: A strategy for enrichment of claudins based on their affinity to Clostridium perfringens enterotoxin

AN Protein MWapp MW SC N R comment
Q63400 Claudin-3 20000 23269 298 6 11.9 tight junction
O35054 Claudin-4 20000 22150 170 3 11.0 tight junction
Q9Z1L1 Claudin-7 20000 22416 157 2 11.0 tight junction
P56745 Claudin-1 20000 22850 171 3 10.2 tight junction
Q8CFI7 DNA-directed RNA polymerase II subunit RPB2 120000 133911 1911 42 7.7 nuclear
P60898 DNA-directed RNA polymerase II subunit RPB9 10000 14532 234 3 7.7 nuclear
Q9ERU9 Ran-binding protein 2# 130000 341091 579 14 7.6 nuclear
P97760 DNA-directed RNA polymerase II subunit RPB3 30000 31311 470 7 6.2 nuclear, cytosolic
Q99M87 DnaJ homolog subfamily A member 3 36000 52443 90 2 6.1 mitochondrial
O15514 DNA-directed RNA polymerase II subunit RPB4 14000 16311 134 2 6.0 nuclear
P62832 60S ribosomal protein L23 14000 14865 296 7 5.5 ribosomal
Q9R112 Sulfide:quinone oxidoreductase 38000 50340 916 18 4.9 mitochondrial
Q5RK28 Normal mucosa of esophagus-specific gene 1 protein 5000 9598 143 3 4.2 nuclear
Q9D6M3 Mitochondrial glutamate carrier 1 27000 34670 153 4 4.1 mitochondrial
Q5I0H3 Small ubiquitin-related modifier 1 (SUMO-1) 85000 11557 147 3 4.1 nuclear, cytosolic
P46061 Ran GTPase-activating protein 1 85000 63616 506 9 4.0 nuclear, cytosolic
P29147 D-beta-hydroxybutyrate dehydrogenase 25000 38202 129 3 3.9 mitochondrial
Q62425 NADH dehydrogenase 1 alpha subcomplex 7000 9327 234 5 3.3 mitochondrial
P13437 3-ketoacyl-CoA thiolase 40000 41871 589 14 3.3 mitochondrial
Q9JIA7 Sphingosine kinase 2 64000 65618 109 2 3.3 cytosolic
Q8BH59 Calcium-binding mitochondrial carrier protein Aralar1 62000 74570 394 8 3.2 mitochondrial
Q66X93 Staphylococcal nuclease domain-containing protein 1 99000 101952 795 18 3.1 nuclear
Q8N163 KIAA 1967 110000 102902 613 12 3.1 nuclear
Q5I0E6 RNA polymerase II-associated protein 2 64000 67892 140 3 3.1 not assigned1
Q925I1 ATPase family AAA domain-containing protein 3 53000 66742 565 12 3.0 mitochondrial
  1. Each protein was identified in at least two of three independent experiments (including one experiment applying inverse labeling). AN, UniProtKB/SwissProt protein accession number; MWapp, apparent molecular weight [Da] according to position in gel; MW, molecular weight [Da] according to UniProtKB/SwissProt; SC, protein (Mascot™) score (highest value of individual experiment); N, highest number of peptides identified in individual experiment; R, ratio between MS peak intensities of peptides originating from GST-CPE116–319 and GST elution fractions; comment, cellular component according to UniProtKB/SwissProt (1nuclear/cytosolic according to [22]); #, protein fragment