Figure 4
From: A strategy for enrichment of claudins based on their affinity to Clostridium perfringens enterotoxin
Sphingosine kinase enrichment in GST-CPE 116–319 eluate fraction and sphingosine kinase immunoprecipitation. Immunoblot (IB) against sphingosine kinase 2 (SphK2) of protein fractions after GST-CPE116–319 affinity purification (upper panel); immunoblots against SphK2 (middle panel) and claudin-3 (lower panel) of protein fractions obtained by SphK2 immunoprecipation (IP); lane 1, lysate of NRC cells; lane 2, flow-through of IP fraction; lane 3, flow-through of control fraction (protein A/G plus-Agarose); lane 4, eluate of IP fraction; lane 5, eluate of control fraction.