Figure 3
From: A strategy for enrichment of claudins based on their affinity to Clostridium perfringens enterotoxin
Claudin enrichment in GST-CPE 116–319 eluate fraction. Immunoblots against claudin-1, claudin-3, claudin-4, claudin-5 and claudin-7 of protein fractions obtained from NRC cells after GST-CPE116–319 affinity purification; lane 1, lysate (positive control); lane 2, flow-through of GST-CPE116–319 fraction; lane 3, flow-through of control fraction (GST only); lane 4, eluate of GST-CPE116–319 fraction; lane 5, eluate of control fraction.