Figure 7

Identification of the different SHBG transcription units in prostatic and non-prostatic cells by RT-PCR. A) PCR amplification of transcription units containing exon 1A and 1B in prostate cancer cell lines resulted in two bands: the expected band (α band) derived from exon 1A/1B-exon 2 junction, and an upper band (β band) resulting from the exon 1A/1B-exon 1 junction. A third band, caused by exon 4 skipping (γ band), was also observed in the amplification of 1A transcripts. B) PCR amplification in non-tumoral tissue obtained from human prostate samples. C) PCR amplification in non-prostate cell lines. Negative controls (Ctrl-) contained water instead of cDNA. s18 rRNA was determined to normalize gene expression. D) Scheme of the 5' end of the exon 1 (capital letters) and its flanking 3' intron sequence (small letters). The two 3' splice sites (a and b) are underlined along with the potential pyrimidine tract. The consensus sequences are marked in red.