Improved long-term expression from helper virus-free HSV-1 vectors packaged using combinations of mutated HSV-1 proteins that include the UL13 protein kinase and specific components of the VP16 transcriptional complex

Table 2 The numbers of β-gal-IR positive cells that costain for NeuN-IR from rats sacrificed at 4 days or 2 months after microinjection of pNFHlac packaged using specific combinations of mutated HSV-1 proteins

Packaging condition	Time after gene transfer	Total β-gal-IR positive cells	β-gal-IR and NeuN-IR positive cells	% costained cells
Δul46&47, ul13g	4 days	199	180	90
Δul46&47, ul13g	2 months	110	98	89
VP16in14, gene12, ul13g	4 days	200	181	91
VP16in14, gene12, ul13g	2 months	78	69	88
VP16in14, gene12	4 days	205	190	93
Δul46&47	4 days	195	179	92
ul13g	4 days	211	189	90
wt	4 days	198	176	89

β-gal-IR was detected using a rabbit anti-E. coli β-gal antibody that was visualized with a fluorescein isothiocyanate-conjugated goat anti-rabbit IgG, and NeuN-IR was detected in the same sections using a mouse monoclonal anti-NeuN antibody that was visualized with a rhodamine isothiocyanate-conjugated goat anti-mouse IgG.

ISSN: 1471-2199