Table 1 Representative primer sequences used in this study
From: A noncoding RNA gene on chromosome 10p15.3 may function upstream of hTERT
Gene | Oligonucleotide | Sequence (5'-3') |
|---|---|---|
RGM249 (for RT-PCR) | sense primer | TGAGAGAATGGGTGTCCAAG |
| Â | antisense primer | CCTGGAAAGTCTAGGGATGT |
RGM249 (for real-time RT-PCR) | sense primer | AGAGAATGGGTGTCCAAGAGG |
| Â | antisense primer | GGACATGTTCAGCTTCCCCTTT |
hTERT | sense primer | CGGAAGAGTGTCTGGAGCAA |
| Â | antisense primer | GGATGAAGCGGAGTCTGGA |
RPL22 (for RT-PCR) | sense primer | ATTGCACCCACCCTGTAGAA |
| Â | antisense primer | TGTTAGCAACTACGCGCAA |
RPL22 (for real-time RT-PCR) | sense primer | GACCATCGAAAGGAGCAAGA |
| Â | antisense primer | TGTTAGCAACTACGCGCAAC |
β-actin | sense primer | TCACCCACACTGTGCCCATCTACGA |
| Â | antisense primer | CAGCGGAACCGCTCATTGCCAATGG |