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Table 2 Melting temperature (°C) of amplification products with standard deviations.

From: Validation of commonly used reference genes for sleep-related gene expression studies

Tissue

Beta-actin

B2M

GAPDH

HPRT

GOI

Brain

81.0 ± 0.2

79.5 ± 0.2

77.7 ± 0.3

78.2 ± 0.2

77.9 ± 0.1

Blood

81.1 ± 0.2

79.6 ± 0.2

77.7 ± 0.3

78.3 ± 0.2

80.1 ± 0.3

  1. GOI: gene of interest (BDNF for brain and GPD1 for blood).