Figure 2
From: Validation of commonly used reference genes for sleep-related gene expression studies
Expression of BDNF in the brain. A. The expression of BDNF was quantified by RTqPCR in the brain of controls (C), animals subjected to paradoxical sleep deprivation for 96 hours (PSD96), and animals subjected to PSD and sleep recovery for 24 hours (SR24). B. The expression of BDNF was also evaluated in animals subjected to total sleep deprivation for 6 hours (TSD6) and compared to the control (C). The relative expression fold changes were calculated using the 2-ddCt method for each reference gene or the normalization factor (NF) generated by GeNorm software. Error bar represents standard error of the mean. * p < 0.05 compared to control, # p < 0.05 compared to PSD (ANOVA followed by Tukey's post hoc test).