Figure 5
From: TCF/β-catenin plays an important role in HCCR-1 oncogene expression
Endogenous expression of HCCR-1 on activated Wnt/β-catenin signal by treatment with the GSK-3β inhibitors, LiCl or AR-A014418. Total RNAs were prepared from K562 (A and B) and HEK/293 (C and D) cells which were treated with various concentration of LiCl (A and C) or AR-A014418 (B and D) for 24 hours in respectively. Five μg of total RNAs were used for cDNA synthesis and subjected to real-time PCR analysis with human specific primers for HCCR-1 and β-actin (mean ± S.D., n = 3).