Figure 1
From: TCF/β-catenin plays an important role in HCCR-1 oncogene expression
Expression of the truncated HCCR-1 promoter in K562, HEK/293, or A549 cells. The DNA constructs containing various lengths of the HCCR-1 promoter region were cloned into upstream of the firefly luciferase (LUC) reporter gene. Each luciferase reporter construct shown in the diagram was co-transfected in K562 (A and B; filled bars), HEK293 (A; open bars), and A549 (A; shadow bars) cells with the pRL-CMV normalizing reporter plasmid encoding with the renilla (REN) gene. The horizontal axis shows the ratio of luciferase to renilla activity normalized by LUC/REN (= 100%) displayed by cells co-transfected with pRL-CMV and pGL3-control. We designated each recombinant vector as pGL3X~Y, where X is the first base and Y the last base of each truncated promoter (n > 5 per construct).