Figure 3
Orf1 activates translation of abiD1 TIR: luxAB fusions. (A) Translation of abiD1 TIR I:luxAB, abiD1 TIR II:luxAB and aldB TIR:luxAB fusions in the absence (control) or in the presence of orf1, orf1 M1 and orf1 M2 genes in L. lactis IL1403 cells. Experiments were performed at 30°C. Results are means of 6 independent experiments. (B) Transcription of abiD1 TIR:luxAB fusions. The amount of abiD1 TIR I: luxAB and abiD1 TIR II: luxAB transcripts was measured by quantitative RT-PCR in the absence (control) and in the presence of orf1 or orf1M1 genes in L. lactis cells grown at 30°C. The aldB TIR: luxAB fusion was used as a control. Amount of transcript was normalized to the L. lactis tuf transcript level. Values are means of 7 – 15 measurements, expressed in arbitrary units. (C) Translation of abiD1 TIR II:luxAB fusion in the presence of plasmid- or chromosome- integrated orf1 and orf1 M2 in B. subtilis 168 cells. Experiments were performed at 30°C. Results are means of 4–5 independent experiments. Luciferase activity is shown in arbitrary light units (lux/OD unit at an OD600 of 0.4).