Figure 5
The expression of liver-enriched transcription factor genes in pALB-EGFP/ES cell line 9 (A.9) cultures. The figure shows the expression levels of HNF-1b (A); HNF-3b (B); HNF-3a (C); C/EBP-b (D); HNF-4a (E) and HNF-6 (F) genes in pALB-EGFP/ES cell line 9 (A.9) cultured in HIM. Data are presented as means ± SEM of three independent experiments (A.9.2+ = GFP-expressing cells purified from cultured A.9 population at culture day 21, A.9.3+ = GFP-expressing cells purified from cultured A.9 population at culture day 23, A.9.3- = GFP-negative cells purified from cultured A.9 population at culture day 23, HNF-1b, HNF-3b, HNF-3a, HNF-4a, HNF-6 = hepatocyte nuclear factor-1β, -3β, -3α, -4α and 6, respectively; C/EBP-b = CCAAT-enhancer binding protein-β, Liver = intact mouse liver). [*P < 0.05 vs. N populations].