Figure 3

RT-PCR analysis for hepatocyte-lineage marker genes in pALB-EGFP/ES cell line 9 (A.9) cultures. The figure shows agarose gel electrophoresis of RT-PCR analysis for HNF-3β, AFP, Albumin, TAT, G6Pase, Cyp3a, TDO and Pepck gene expression in pALB-EGFP/ES cell line 9 (A.9) cultured in HIM [N = mES cell populations generated from HIM culture, Number indicate days in culture (as described in materials & methods), A.9.2+ = GFP-expressing cells purified from cultured A.9 population at culture day 21, A.9.3+ = GFP-expressing cells purified from cultured A.9 population at culture day 23, A.9.3- = GFP-negative cells purified from cultured A.9 population at culture day 23, HNF-3b = hepatocyte nuclear factor-3β, AFP = α-fetoprotein, Cyp3a = cytochrome P450-3a, G6Pase = glucose-6-phosphatase, Pepck = phosphoenolpyruvate carboxykinase, Pou5f1 = POU domain class5 transcription factor 1, TAT = tyrosine aminotransferase, TDO = tryptophan 2,3-dioxygenase, Liver = intact mouse liver].