Table 1 Strains and plasmids used or generated in this study.
From: Use of the λ Red-recombineering method for genetic engineering of Pantoea ananatis
Name | Main characteristics/accession number | Source or reference |
|---|---|---|
Strains | ||
Pantoea ananatis SC17 (AJ13355) | mutant with decreased secretion of mucus | [1] |
Pantoea ananatis SC17(0) | Derivative of SC17 resistant to expression of λ Red genes | This work |
E. coli K12 MG1655 | Wild type | VKPM |
Plasmids | ||
RSF1010 | GenBank accession number NC_001740 | [26] |
pUC4K | GenBank accession number X06404 | [32] |
pMW-attL λ - KmR-attR λ | Donor attL λ - KmR-attR λ cassette; ApR; KmR | [35] |
pKD46 | pINT-ts; λ gam, bet, and exo genes are under P araB promoter; ApR | [4] |
pRSFRedTER | λ gam, bet, and exo genes are under control of P-element; sacB gene; CmR | This work |
pRSFRedkan | λ gam, bet, and exo genes are under control of P-element; KmR | This work |
pRSFGamBet | λ gam and bet genes are under control of P- element; sacB gene; CmR | This work |
pRSFGamBetkan | λ gam and bet genes are under control of P- element; KmR | This work |
pRSFPlacsacB | PlacUV5-sacB-cat cassette; CmR | This work |
pMW-intxis-cat | pSC101-ts; λ xis-int genes transcribed from λ PR promoter under CIts857 control; CmR | This work |