Table 1 Plasmids and primers used in this study
Plasmid ** | Primers | Sequence 5' – 3'a | Origin |
|---|---|---|---|
T25-Erp | Erpbaitup | ctgcag gggtgccgaaccgacgccga | This study |
ERPbaitlow | ggtacc ttaggcgaccggcacggt | ||
T25-1417 | 1417pktup* | ggatcc cgtgaccgccgcaccgaac | This study |
1417pktlow* | ggtacc tcagcgggcgcacaggtc | ||
T25-2617 | 2617pktup* | ctgcag ggatgagcatcagaccaacg | This study |
2617pktlow* | ggtacc ttaaggccgcccgatgcc | ||
T25-MlErp | ERPlepraeup | ggatcca gtgccgaaccgacgccgatg | This study |
ERPlepraelow | ggatcc ctacgtgacaggaatcagtg | ||
T25-MsErp | ERPsmgupok | ggtacc tgtgccgaaccgccgtcga | This study |
ERPsmglow | ggtacc tcagggtcgccgcgat | ||
T18- ErpA | ERPputup | ctgcag gggtgccgaaccgacgccga | This study |
ERPaminochicolow | ggtacc ctaggtcaggctgggcaccgg | ||
T18-PGLTS | ERPPGLTSUp | ctgcag ggggagcggcgatgccagcac | This study |
ERPPGLTSlow | ggtacc ctattcgttggcgccgcccag | ||
T18-ErpC | ERPCup | ctgcag gatcccgattacgacgccggt | This study |
ERPputlow | ggtacc ttaggcgaccggcacggt | ||
T18-Erp | ERPputup | ctgcag gggtgccgaaccgacgccga | This study |
ERPputlow | ggtacc ttaggcgaccggcacggt | ||
T18-ErpΔC | ERPputup | ctgcag gggtgccgaaccgacgccga | This study |
ERP A low | ggtacc ctattcgttggcgccgcccag | ||
pmip12-1417-myc | up1417myc | ggtacc gtgaccgccgcaccga | This study |
LowMyc | ggtacc ttagtgatggtgatggtg | ||
pmip12-2617c-myc | up2617cmyc | ggtacc atgagcatcagaccaac | This study |
LowMyc | ggtacc ttagtgatggtgatggtg | ||
pmip12 | - | - | [26] |
ImpactVector 1.1 | - | - | Wageninger UR |
- | LeftOP1417*** | cagctggcacggaaagat | This study |
RightOP1417*** | acgacacaccgatcacttca |