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Figure 4 | BMC Molecular Biology

Figure 4

From: Identification of two proteins that interact with the Erp virulence factor from Mycobacterium tuberculosis by using the bacterial two-hybrid system

Figure 4

Cellular localization of Rv1417, Rv2617c and Erp. Myc-Rv1417 and Myc-Rv2617c were expressed in M. smegmatis and M. tuberculosis, respectively. Proteins from culture filtrates (CF), cytoplasm©, plasma membrane (M) and cell wall (CW) were extracted from the strains indicated above each lane and separated by 12% SDS-PAGE. Bands were detected either with anti-Myc Mab (Sigma-Aldrich) at a 1:100 dilution (A and B) or with anti-P36/Erp Mab [5] at 1:500 dilution©. MT: M. tuberculosis, MT (pmip12-2617c-myc): M. tuberculosis (pmip12-2617c-myc), MT (pmip12): M. tuberculosis (pmip12), MS (pmip12-1417-myc): M. smegmatis (pmip12-1417-myc), MS (pmip12): M. smegmatis (pmip12), MB P36: M. bovis ΔP36.

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BMC Molecular Biology

ISSN: 1471-2199

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