Table 2 Primers used for cloning and expression studies
From: Functional characterization and identification of mouse Rad51d splice variants
Primer Name | Sequence |
|---|---|
Rad51d F1 | 5'-GCTGACTTGGAGGAAGTAGCCCAGAAGTGT-3' |
Rad51d F2 | 5'-CTACTTGATGCTGGCCTCTATACTGG-3' |
Rad51d F3 | 5'-GCAGGAAGCAACTTCTTCAGGCG-3' |
Rad51d R1 | 5'-AGCCTGTAGTAGCTGGAGGAGG-3' |
Rad51d R2 | 5'-TGAACGCACCACCTGTATCCTCTGGAGAG-3' |
Rad51dΔ9,10 R1 | 5'-GTTCTAAGACAGACAGAGCAC-3' |
Rad51d Δ10 R1 | 5'-GAGACACAGGTTCTTCACCACAC-3' |
Rad51dΔ8StuI | 5'-TGACCAACCACTTGACTCGAGATTGGGATGGTAGG-3' |
Rad51dΔ8BamHI | 5'-GATCCCTACCATCCCAATCTCGAGTCAAGTGGTTGGTCA-3' |
Rad51dΔ7b F1 | 5'-GCCCCACTTCTGGGAGGCCTGGCCTTGATG-3' |
Rad51DΔ7b R1 | 5'-CATCAAGGCCAGGCCTCCCAGAAGTGGGGC-3' |
Rad51dΔ7,8AvaII | 5'-GACCTTCGCGGCACCATAGCCCAGCAGGTGACCAACCACTTGAC-3' |
Rad51dΔ7,8XhoI | 5'-TGCAGTCAAGTGGTTGGTCACCTGCTGGGCTATGGTGCCGCGAAG-3' |
Rad51dΔ3BstXI | 5'-GTGGCTTGTCCTACAAGCCTGGACAAACTACTTGAG-3' |
Rad51dKpnIF | 5'-GGACTATGGGTACCCTCAGGGCA-3' |
Rad51dΔ5NheBamR | 5'-CAGGATCCAGCTAGCCTGGGTTTTGCC-3' |
Rad51dΔ5NheF | 5'-AACTCAGGCTAGCGCTCTCCAGAGG-3' |
Rad51dBamR | 5'-CAGTGGATCCCAATCAACAGTGTCA-3' |
Rad51d+int3F | 5'-CCGGCATCGGAAGGTTTGTATGCGAACCTGGACAAGCCTGGACAA ACTACTTGATGCTGGCCTCTATACTGGGGAAGTGAG-3' |
Rad51d+int3R | 5'-GATCCCTCACTTCCCCAGTATAGAGGCCAGCATCAAGTAGTTTGTC CAGGCTTGTCCAGGTTCGCATACAAACCTTCCGATG-3' |
Rad51dex6F | 5'-CCAGAGGATACAGGTGGTGCGTTCATTTGAC-3' |
Rad51dΔ9,10BamR | 5'-CCGGGATCCGTTCTAAGACAGACAGAGCAC-3' |
Rad51dΔ10BamR | 5'-CCGGGATCCGAGCACAGGTTCTTCACCA-3' |
Rad51cKpnF | 5'-CTTGGTACCCAGCGGGAGTTGGTGGGT-3' |
Rad51cBamR | 5'-CAGTTAACTGGATCCACTGGCA-3' |
XRCC2F | 5'-ATGCTACGGCTCGTGACAGTTCTT-3' |
XRCC2R | 5'-AGAAGATGACCCTGTGCTTCACGA-3' |