Figure 7
From: HMGB1/2 can target DNA for illegitimate cleavage by the RAG1/2 complex
HMGB1 exhibits preferential binding to bps6197 relative to a 23-RSS. (A-B). Radiolabeled oligonucleotide substrates containing a consensus 23-RSS or bps6197 were incubated under binding conditions with increasing amounts of (A) full-length HMGB1 (0–320 ng in 40 ng increments; approximate protein:DNA ratio of 0–642:1) or (B) tailless HMGB1 (0–140 ng in 20 ng increments; approximate protein:DNA ratio of 0–328:1). Protein-DNA complex formation was analyzed by EMSA as in Figure 3B.