Figure 7

PAT does not require ATP for relaxation of supercoiled DNA. All reactions contain 110 ng of supercoiled pUC18 and 9 ng of PAT. R indicates relaxed DNA and S indicates supercoiled pUC18 in lane 1. Lane 2 contains 0.1 mM ATP and 0 mM MgCl₂, lane 3 contains 1 mM ATP and 0 mM MgCl₂, lane 4 contains 0.1 mM ATP and 0.25 mM MgCl₂, lane 5 contains 1 mM ATP and 0.25 mM MgCl₂, lane 6 contains 0.1 mM ATP and 2.5 mM MgCl₂, lane 7 contains 1 mM ATP and 2.5 mM MgCl₂.