Identification of novel Bach2 transcripts and protein isoforms through tagging analysis of retroviral integrations in B-cell lymphomas

Table 1 Summary of proviral integration in NMRI-i mice into the Bach2 gene

No.	Mouse ID	Virus variant^a	Region	Orientation^b	PCR^c	Southern blots^d
S1	03–655	Akv 1–99Runx	5' promoter	-	+	-
S2	99–64	Akv PBS-Gln	5' promoter	-	+	-
S3	99–148	Akv PBS-Gln	Intron1	-	+	-
S4	01–1124	Akv 1–99mRunx+Egre	Intron1	+	+	n.d.
S5	98–1286	Akv PBS-Gln	Intron1	-	+	n.d.
S6	99-97	Akv PBS-Gln	Intron1	-	+	-
S7	01–454	Akv 1–99wt	Intron1	-	+	-
S8	98–1197	Akv PBS-Pro	Intron1	-	+	-
S9	99–955	Akv 1–99wt	Intron2	-	+	-
S10	99–1020	Akv 1–99wt	Intron2	-	+	-
S11	99–1206	Akv 1–99wt	Intron2	-	+	-
S12	03–858	Akv 1–99mGR	Intron2	-	+	-
S13	99–128	Akv PBS-Lys	Intron2	-	+	-
S14	99–128	Akv PBS-Lys	Intron2	-	+	-
S15	03–653	Akv 1–99mEgre	Intron2	-	+	-
S16	99-74	Akv PBS-Gln	Intron3	+	+	-
S17	03–290	Akv/SL3-3 TM	Intron3	+	+	n.d.
S18	99-95	Akv PBS-Arg	Intron3	-	+	-

^aThe virus variants originated from our published [22, 24, 25, 30, 56–60] or unpublished pathogenicity work. ^bThe virus was transcribed in the same (+) or opposite (-) orientation compared to that of the mouse Bach2 gene. ^cProvirus was detectable (+) or undetectable (-) by PCR. ^dSouthern blots showed the proviral integration to be present in a minor fraction of cells in tumor tissue (-). N.d., not determined.

ISSN: 1471-2199