Figure 4

Expression level analysis of Bach2 mRNA derived from the normal or alternative promoter. Quantitative RT-PCR analyses were performed with primer pairs amplifying either cDNA representing transcripts derived from the normal Bach2 promoter (primers P1F and Q3R) or cDNA representing transcripts derived from the alternative promoter (primers 2F and 2R). RNA was extracted from NMRI mice spleen (Spl) or tumor 1206 material and cDNA generated using either random-primed first strand synthesis (panel A) or oligo dT primed first strand synthesis (panel B). Expression levels were normalized to the tbp expression level and the expression levels for the normal Bach2 transcript in NMRI spl set to 20.