Figure 2

Expression analysis screened out a Bach2 integration site with high mRNA expression level. Q-PCR was performed on total RNAs from tumors with (asterisked) or without proviral integration in Bach2. The used primer pairs and their schematic localization is shown in (A), (B), (C), (D), and (E) (for localization and origin of exon 2A, see figure 5 and text). M, N, and S (mesenteric, cervical (neck) lymph node, and spleen, respectively) refer to types of tumor tissues used for the analyses. Bach2 expression levels were normalized to the tbp expression level and the Bach2 expression level in spleen tissue from untreated NMRI mice (NMRI S) was given the value 20.