Table 2 The primers used for the bCSH1 (NW_001494181) 5'-flanking region of the sequencing and reporter constructs.
Position | Strand | Sequence | Annealing |
|---|---|---|---|
-4090 to -4073 | Forward | 5'-ATGGTACCATTGTCTATTACAGGGTGCA-3' | 65.5 |
-599 to -581 | Forward | 5'-GGGGTACCCCTGTCCTAGTTCTTTAAC-3' | 64 |
-368 to -353 | Forward | 5'-GGGGTACCCCTTAGATCTCTGAGTAG-3' | 62.6 |
-213 to -194 | Forward | 5'-GGGGTACCCCATAGGGTGTATACAGATAC-3' | 62 |
-8 to +8 | Reverse | 5'-ATCTCGAGATGGGAATGCCTAAGGAG-3' | Â |