Table 1 Funcional classification and Gene Ontology (GO) terms over-represented in biological process for the 177 selected wheat candidate reference genes.
From: Identification and validation of reference genes for quantitative RT-PCR normalization in wheat
| Â | Functional Class | Representations (%) | GO terms | Biological process | NS (GO) | (%FC) | (%TS) |
|---|---|---|---|---|---|---|---|
1) | Biological function not annotated | 34 (19.21) | Â | Â | Â | Â | Â |
2) | Cellular organization | 32 (18.08) | GO:0015031 | Protein transport | 15 | 46.88 | 8.47 |
| Â | Â | Â | GO:0006810 | Transport | 8 | 25.00 | 4.52 |
3) | Metabolism | 31 (17.51) | GO:0006520 | Amino acid metabolic process | 7 | 22.58 | 3.95 |
| Â | Â | Â | GO:0006007 | Glucose catabolic process | 6 | 19.35 | 3.39 |
| Â | Â | Â | GO:0006099 | Tricarboxylic acid cycle | 5 | 16.12 | 2.82 |
4) | Posttranslational modification (protein turnover) | 28 (15.82) | GO:0006508 | Ubiquitin-dependent protein catabolic process | 15 | 53.57 | 8.47 |
| Â | Â | Â | GO:00016925 | Proteolysis | 8 | 28.57 | 4.52 |
5) | Protein synthesis | 16 (9.04) | GO:0006413 | Translation initiation | 8 | 50.00 | 4.52 |
| Â | Â | Â | GO:0042254 | Ribosome biogenesis and assembly | 6 | 37.50 | 3.39 |
6) | Signal transduction | 13 (7.34) | GO:0007243 | Protein kinase cascade | 5 | 38.46 | 2.82 |
7) | RNA processing | 7 (3.96) | Â | Â | Â | Â | Â |
8) | Energy | 6 (3.39) | GO:0022900 | Electron transport | 6 | 100 | 3.39 |
9) | Transcription | 5 (2.83) | GO:0045449 | Regulation of transcription | 5 | 100 | 2.82 |
10) | Biotic and abiotic stress related | 3 (1.69) | Â | Â | Â | Â | Â |
11) | Development | 2 (1.13) | Â | Â | Â | Â | Â |