Figure 3
From: Identification and validation of reference genes for quantitative RT-PCR normalization in wheat
Expression level of 32 candidate reference genes in 24 tissues, developmental stages and seedlings exposed to high and low temperatures. The expression levels (Ct values) of each candidate reference gene (UniGene cluster) are shown as average (diagonal cross), median (horizontal lines), 25th to 75th percentile (boxes) and expression ranges (whiskers) for the 24 cDNA pools analysed. The line at Ct 22.31, corresponding to the general mean, discriminates the reference genes with high and low expression.