The expression of CPEB3 proteins in the retina. a) The regulation of CPEB3 protein during the postnatal development of the retina. CPEB3 western blot on retinal samples from seven different postnatal ages showed that the 75 kD band protein was increased during the development. Another band at ~130 kD was also present in the retina. Both bands diminished when the antibody was pre-adsorbed with the recombinant CPEB3 protein (the last lane). GAPDH was used as a loading control. b) Both of the 75 kD and the 130 kD bands diminished when Hela cells were transfected with CPEB3 siRNA. Cells in the negative control were treated with a negative control siRNA. GAPDH was used as a loading control.