Figure 6

In vivo effects of deletion of exon 6A nucleotides 22-30 on splicing in reporter construct. Mice (n = 3/group) were injected by high volume tail vein method with 1.6 ml of PBS containing 100 μg of plasmid. After 2 days, mice were sacrificed and liver was recovered and used for RNA preparation. Total RNA was treated with DNaseI to avoid the contamination by genomic DNA A. One-step RT-PCR analysis of splicing pattern. Each lane represents RT-PCR product from a different mouse. The anticipated positions of the products for the splicing events with inclusion or exclusion of exon 6A are indicated by arrows. MW = lane with molecular weight markers. B. The exon 6A inclusion percentage was calculated from densitometric scan of gels as described for Figure 4, and the mean ± standard deviation is plotted for each group.