BMC Molecular Biology

Table 1 PCR primer pairs.

From: Removal of Hsf4 leads to cataract development in mice through down-regulation of γS-crystallin and Bfsp expression

Gene name	Primers	Annealing temperature
hHSF4F	CCCCCTCAAGAAAGTGTGGAA	60°C
hHSF4R	GAACCAAGGGCTGCATCAAG	60°C
mHSF4F	GGCACCAGCTTCCTCGTAAG	60°C
mHSF4R	CCTTCCGAAAACCATACATGTTG	60°C
hCrygsF	CCCAACTTTGCTGGGTACATG	60°C
hCrygsR	GGCCTCCACTAGGCAGATGA	60°C
mCrygsF	AGCGTTGGATGGGCCTTAAT	60°C
mCrygsR	GTTTCGTACATCTGACCGTTGAAG	60°C
hBFSP1F	TTCAGTGGGAGAGAGATGTTGAAA	60°C
hBFSP1R	CTCCTGGGTCTGGGACATGT	60°C
mBfsp1F	AGACGCCCATCTCTCTGATCA	60°C
mBfsp1R	AGGGCCTTCTGTCTCGGTTT	60°C
hBFSP2F	TTCAGTGGGAGAGAGATGTTGAAA	60°C
hBFSP2R	CTCCTGGGTCTGGGACATGT	60°C
mBfsp2F	GCACCGGTCTGGATGATGTC	60°C
mBfsp2R	ACGTGGACCACCTCTGTCTG T	60°C
mLp82F	ATTGGCTTCGCCATCTACGA	60°C
mLp82R	CCCGCATGTTGATGTAGGTTT	60°C
mCalpain2F	ATGCGGAAAGCACTGGAAGA	60°C
mCalpain2R	CCAAACACCGCACAAAATTG	60°C

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ISSN: 1471-2199

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