Figure 2
From: A simple method for generating full length cDNA from low abundance partial genomic clones
Full length HOXD13 cDNA PCR product analysis. 1) 1.1 kb full length homeobox D13 cDNA generated by using DF1 and DR2 specific primers. 2 and 3) 100 bp ladder (Gibco BRL Life Technologies, Rockville, MD) 4). Restriction endonuclease digestion of PCR product with Ppum 1 showing correct expected size fragments of 519, 316 and 265 bps. respectively 5). 1 kb ladder (Gibco BRL Life Technologies, Rockville, MD) 6-8). are restriction digestion fragments of full length HOXD13 cloned in PCR II (vector) with Eco R1 (1.1 kb), Kpn 1 (381 bp) and Sma 1 and Bam H1 (826 bp) respectively.