Figure 2From: ABCA1, ABCG1 and SR-BI: hormonal regulation in primary rat hepatocytes and human cell linesEffects of dexamethasone, insulin, or mifepristone in HepG2 cells. HepG2 cells were incubated with the indicated concentrations of dexamethasone (Dex), insulin (Ins), or mifepristone (Mif) in lipid-deficient medium for 24 hours and examined by real time RT-PCR and Western blotting. A. The relative mRNA expression of SR-BI (black bars), ABCA1 (white bars) and ABCG1 (gray bars). B. Representative Western blots of ABCA1, ABCG1, SR-BI and β-actin. Proteins (50 μg) were separated on a 7.5% polyacrylamide gel as described in materials and methods. C. The relative mRNA expression of CYP7A1 (black bars) and apoA-I (white bars). The results were normalized to GAPDH mRNA expression and expressed relative to the non-treated controls (medium B only) (± STDEV). The experiments were performed at least three times. * (p < 0.05) indicate significantly difference from control.Back to article page