Figure 5
Phenotypic analysis of FACS-isolated ES cell clones. Panels A-C show images of three clones with different phenotypes. (A) colony of cells expressing CFP only, (B) colony of cells expressing YFP only, (C) colony of cells expressing both CFP and YFP. Images shown were are produced by merging three images, CFP epifluorescence, YFP epifluorescence, and phase contrast. Gray cells under the ES clones are a monolayer of non-fluorescent wild type MEF feeder cells. Arrows indicate some of the cellular debris and dead cells that exhibited autofluorescence. Panels D, E and F correlate with panels A, B and C, respectively, and show FACS analysis of each subclone. Polygons indicate gates used to collect putative monochromatic cells. Arrows indicate residual MEF feeder cells present in ES cell populations subjected to FACS.