Expression and loss of alleles in cultured mouse embryonic fibroblasts and stem cells carrying allelic fluorescent protein genes
© Larson et al; licensee BioMed Central Ltd. 2006
Received: 15 June 2006
Accepted: 16 October 2006
Published: 16 October 2006
Loss of heterozygosity (LOH) contributes to many cancers, but the rate at which these events occur in normal cells of the body is not clear. LOH would be detectable in diverse cell types in the body if this event were to confer an obvious cellular phenotype. Mice that carry two different fluorescent protein genes as alleles of a locus would seem to be a useful tool for addressing this issue because LOH would change a cell's phenotype from dichromatic to monochromatic. In addition, LOH caused by mitotic crossing over might be discernable in tissues because this event produces a pair of neighboring monochromatic cells that are different colors.
As a step in assessing the utility of this approach, we derived primary embryonic fibroblast populations and embryonic stem cell lines from mice that carried two different fluorescent protein genes as alleles at the chromosome 6 locus, ROSA26. Fluorescence activated cell sorting (FACS) showed that the vast majority of cells in each line expressed the two marker proteins at similar levels, and that populations exhibited expression noise similar to that seen in bacteria and yeast. Cells with a monochromatic phenotype were present at frequencies on the order of 10-4 and appeared to be produced at a rate of approximately 10-5 variant cells per mitosis. 45 of 45 stably monochromatic ES cell clones exhibited loss of the expected allele at the ROSA26 locus. More than half of these clones retained heterozygosity at a locus between ROSA26 and the centromere. Other clones exhibited LOH near the centromere, but were disomic for chromosome 6.
Allelic fluorescent markers allowed LOH at the ROSA26 locus to be detected by FACS. LOH at this locus was usually not accompanied by LOH near the centromere, suggesting that mitotic recombination was the major cause of ROSA26 LOH. Dichromatic mouse embryonic cells provide a novel system for studying genetic/karyotypic stability and factors influencing expression from allelic genes. Similar approaches will allow these phenomena to be studied in tissues.
During malignant progression, cells accumulate multiple genetic and epigenetic alterations that cause loss of at least one anti-oncogenic function. Such a loss can be caused by a variety of events including mutation and losses that take place at the chromosome level, e.g. loss of heterozygosity (LOH), which is a hallmark of numerous cancers [1–5]. Many cases of LOH are caused by mitotic recombination (MR) between homologous chromosomes . LOH can also arise via uniparental disomy (UPD), a change that presumably begins with nondisjunction of sister chromatids, producing trisomy in a daughter cell. Subsequent mis-segregation during mitosis of a trisomic cell can produce a disomic cell where both homologues were derived from the same parental homologue (UPD) . On other occasions, gene conversion (GC) and interstitial deletions cause LOH [8, 9]. In addition, it has recently come to light that some cells in the brain can be monosomic for one or more chromosomes .
Tumors serve as indicators of allele loss, but not all allele loss events necessarily lead to a tumor. LOH in diverse, nontransformed cell types in the body would be directly detectable if this event were to confer an obvious phenotype (other than tumorous growth) on an individual cell, its sibling, and their progeny. Mice that carry two different fluorescent protein genes as alleles of a locus would seem to be a useful tool for addressing this issue because LOH would change a cell's phenotype from dichromatic to monochromatic. If tissue architecture permits, the cause of LOH would be suggested by the number and arrangement of mutant cells because LOH caused by mitotic crossing over produces a pair of neighboring monochromatic cells expressing different colors . By contrast, LOH caused by other events, such as UPD, gene conversion, or point mutation would be expected to produce a single monochromatic cell.
As a first step in assessing the utility of the allelic marker approach in mammals, we derived cell lines from mice that carried two different fluorescent protein (cyan and yellow) genes as alleles at the widely expressed ROSA26 locus, which is on chromosome 6 . Although our studies were primarily motivated by an interest in LOH in mouse tissues, studies on genetic stability and allelic gene expression in mouse ES cells are of interest in their own right. The totipotent nature of ES cells has made them a useful tool for manipulating the genome and a promising prospect for human therapeutic applications. However, introduction of genetically damaged ES cells could lead to adverse outcomes.
The genetic and karyotypic stability of ES cells in general is not entirely clear. On one hand, aneuploid mouse ES cell lines are fairly common [13, 14]. On the other hand, hundreds of mice have been made from ES cells, showing that these cells can maintain genetic stability when handled properly . Some studies have suggested high rates of allele loss in ES cells [5, 16], while in others, loss rates were hundreds of fold lower . Different rates of point mutation have also been reported for ES cells [17, 18]. The reasons for these different observations are not clear, but could include differences in marker genes employed, methods used to detect variant cells, cell lines studied, rates at which different chromosomes undergo either nondisjunction or mitotic recombination, and inadvertent selection of cells that proliferate better in culture. The bichromatic biallelic ES cells described herein differ from others studied because cells with variant phenotypes can be identified and isolated by FACS.
Fluorescence phenotypes of cells in populations of biallelic embryonic fibroblasts
The two lines of transgenic mice used to make embryonic cell lines were a gift from F. Costantini, whose work had shown that both fluorescent proteins were simultaneously widely expressed in mice, which appeared normal . Three R26CY mouse embryonic fibroblast (MEF) populations were independently derived, each from one 13.5 day post coitus (dpc) embryo. These cells were cultured for a few passages and then subjected to FACS analysis.
Fluorescence phenotypes of cells in populations of biallelic ES cells
Two R26CY ES cell lines were independently derived and studied. Figures 1D and 1E show FACS data obtained from these two cell lines. In addition to the points produced by ES cells, the FACS plots contained a relatively small number of points produced by autofluorescence of the feeder cells present in the ES cell cultures.
The fluorescent phenotypes in the two ES cell lines were similar to those seen in the three R26CY MEF cell lines (described above) except that the fluorescence emitted by each protein in a typical ES cell was about a third as intense as that seen in a typical R26CY MEF cell. Nearly all of the ES cells in each population exhibited both CFP and YFP fluorescence (Figures 3B). In a given cell, normalized CFP and YFP signal intensities tended to be approximately equal, but coordination of expression was not perfect. The two ES cell lines, R26CY2, exhibited intrinsic noise levels of 0.2, similar to that exhibited by R26CY MEFs.
Noise is expected to produce cells that exhibit CFP and YFP fluorescence intensities that differ over time. To determine if this were the case, a population in which the cells were two fold brighter with respect to CFP than YFP was obtained by FACS, placed in culture and passed 3 times. Analysis by FACS showed that the original phenotype (brighter CFP) was not maintained. Instead the population resembled those shown in Figure 3. Hence, the phenotype of the population was transient, as would be expected if it were due to noise.
Spontaneously arising stable monochromatic ES cells lacked the gene encoding the non-expressed fluorescent protein
Analysis of a centromeric marker in cells with LOH at ROSA26
To investigate the nature of the events that produced allele loss at the ROSA26 locus, a centromeric heterozygous microsatellite (D6Mit159) was identified. The D6Mit159 locus is 30 Mbp from the centromere and 83 Mbp from the ROSA26 locus. Heterozygosity was retained at the D6Mit159 locus in 12 of 20 spontaneous monochromatic clones examined, suggesting that 60% of spontaneous monochromatic clones were produced by mitotic crossovers within the 83 Mbp interval between the D6Mit159 microsatellite marker and ROSA26. In the other 8 monochromatic clones, heterozygosity was lost at the D6Mit159 locus. Such a result was consistent with loss of one homologue of chromosome 6, although LOH at both D6Mit159 and ROSA26 might have been caused by mitotic recombination taking place in the 30 Mb interval between the centromere and D6Mit159.
Copy number of chromosome 6 in parental ES cell lines
UPD would explain the monochromatic cells that were disomic for chromosome 6 yet had LOH near the centromere. Development of UPD would be facilitated by trisomy for chromosome 6 in the parental cell lines. Therefore, it was of interest to determine if either of the parental R26CY ES cell lines had this karyotype. To that end, 66 cells in cell line R26CY1 were subjected to spectral karyotyping, and 42 cells in cell line R26CY2 were analyzed by whole chromosome painting. All cells analyzed contained 2 copies of chromosome 6. These data established with 95% confidence that the fraction of cells with trisomy 6 was 5% or less in cell line R26CY1 and 7% or less in cell line R26CY2.
Frequency of monochromatic ES cells and estimated rate of LOH
The original FACS experiments showed that monochromatic variants occurred at frequencies of 3.1 × 10-4 and 2.6 × 10-4 in the R26CY1 and R26CY2 ES cell lines, respectively. These data were acquired from populations of cells that had been derived from blastocysts and kept in culture for several months. Thus, it was possible that the frequencies of monochromatic variants were inflated by their accumulation over time. To examine the relationship between frequencies of monochromatic cells and the rate at which they arise, preexisting monochromatic cells were removed from populations of R26CY cells by FACS. These dichromatic cell populations were expanded for 10 population doublings in culture, and then subjected to FACS analysis. Monochromatic cells were present on the order of 10-4 in both cell lines. Monochromatic cells expressing only blue were approximately as frequent as those expressing only yellow.
Variants can be either more of less frequent than otherwise dictated by their rate of formation if they proliferate more or less rapidly than parental cells. To determine if monochromatic cells might proliferate more rapidly than their dichromatic parents, growth kinetics of clonal populations of different fluorescent phenotypes were studied. The different clonally derived populations exhibited a variety of growth rates, but fluorescent phenotype and growth rate were not correlated (data not shown). In addition, an increase in proliferation rate upon loss of one fluorescent protein seems an improbable scenario for two reasons. First, monochromatic cells did not exhibit less fluorescent signal than dichromatic cells, suggesting that the amount of fluorescent protein in monochromatic cells was not less than in dichromatic cells. Second, although it has been reported that it is possible to cause ill effects by over expressing GFP , there is little evidence of general toxicity associated with fluorescent proteins, which have been used in many ES cell lines [23–27] and in transgenic mice, which are often generated from ES cells expressing one fluorescent protein or another [12, 24–29]. In the case of mice carrying CFP and YFP genes at ROSA26, the animals are viable and reproduce normally, as do mice homozygous for either CFP or YFP at ROSA26 .
These data suggested that the frequencies of monochromatic cells present in populations of R26CY cells reflected the rate of their production and that the two types of monochromatic variants arose at essentially the same rate, which can be estimated from the relationship between frequency of variants and the number of reproductive cycles (generations) the population has undergone, where rate equals the proportion of variants in a final culture divided by the number of generations that have elapsed [30, 31]. By this calculation, the rate of LOH in both cell lines was approximately 10-5 per cell-generation.
Induction of mutation in bichromatic ES cells
Because all 45 spontaneous monochromatic ES cell clones examined exhibited LOH at the ROSA26 locus, it was of interest to determine if LOH were the only pathway capable of producing this phenotype. Therefore, R26CY1 ES cells were treated with ethylmethanesulfonate (EMS), which is a strong inducer of point mutations. The cell population exposed to EMS exhibited 1.7 fold more monochromatic cells as assessed by FACS followed by microscopy.
Mutations produced by EMS in monochromatic ES cell clones.
Predicted protein alteration
R26CY 1025 C-8
1 bp deletion
Truncated after 4th amino acid
R26CY 1013 Y-1
G to A
R26CY 1025 Y-1
G to T
Populations of biallelic dichromatic mouse embryo cells contained numerous cells in which fluorescence of CFP and YFP were not equivalent. In about one in ten thousand cells, CFP and YFP fluorescence were highly disproportionate. This phenotype arose by the spontaneous loss of the gene encoding either CFP or YFP. The number of cells with this type of LOH could be determined by a combination of FACS followed by microscopic observation of populations originating from individual sorted cells.
While rare mutant cells exhibited highly disproportionate fluorescence, similar but nonequivalent CFP and YFP fluorescence was seen in most of the cells in biallelic dichromatic populations. These variations in relative fluorescence were transient and presumably produced by expression noise.
Genetic instability in mouse ES cells
The rate of LOH inferred from the frequency of monochromatic ES cells is similar to rates reported in most other studies on LOH in mouse ES cells. LOH rates at 11 different mapped loci each carrying an inserted neo gene have been reported to range between 10-3 and 10-5 events per cell generation [5, 16, 32]. Experiments on cells carrying neo genes inserted at unknown loci produced similar results . Studies using other markers also reported rates of LOH within the range suggested by the frequency of monochromatic cells [34, 35]. In contrast to the rates reported in most studies, experiments using ES cells heterozygous at the Aprt locus indicated that spontaneous LOH occurred at a rate of approximately 1 × 10-7 events per cell generation . It is not clear why the rate of allele loss at the Aprt locus differed from those seen at other loci, but it is possible that chromosome 8 behaves differently from other chromosomes in this respect.
In mouse ES cells heterozygous at the Aprt locus, about 40% of LOH events had occurred via mitotic recombination and UPD was the most common genetic change associated with LOH at Aprt . Similar results were reported for the FasI locus, where a third of the cells with LOH were produced by mitotic recombination . Lefevbre et al showed that LOH with respect to integrated neo genes was accompanied by LOH at linked markers, but did not attempt to distinguish between mitotic recombination and UPD as the cause of these events .
Our studies on biallelic dichromatic mouse ES cells suggest that LOH at ROSA26 occurred principally by mitotic recombination occurring between ROSA26 and the DMit159 marker that is 30 cM from the centromere. However, because a heterozygous locus telomeric to ROSA26 could not be found, it is not possible to exclude interstitial deletion encompassing the ROSA26 locus but too small to produce an obvious decrease in the size of chromosome 6. Nevertheless, interstitial deletion seems an unlikely contributor because such events rarely generate spontaneous LOH in ES cells [6, 36–39].
About 40% of the cells had LOH at both D6Mit159 and ROSA26. This genotype might have been caused by mitotic recombination taking place in the 30 Mb interval between the centromere and D6Mit159. However, the frequency of monochromatic clones with LOH at both D6Mit159 and ROSA26 was two fold higher than would be expected to be produced solely by mitotic recombination, assuming these events occur in proportion to the distance between markers. We would expect recombination in the 30 Mbp interval between the centromere and the D6Mit159 locus to occur 36% (30/83) as frequently as recombination in the 83 Mbp interval between the D6Mit159 locus, where recombination produced 12/20 (60%) LOH events. Therefore, only 4 of the 8 clones with LOH at D6Mit159 would seem to be attributable to mitotic recombination. Mechanisms other than mitotic recombination that might have contributed to the development of LOH at both loci include UPD and monosomy. UPD seems more likely because monosomy is very rarely seen in mammalian cells and has not been seen at all in mouse ES cells [36, 39].
Potential of biallelic fluorescent markers for studies on genetic instability
Findings obtained in these studies on biallelic dichromatic ES cells suggest that this approach can be extended to tissues isolated from mice. GFP has been shown to be useful for detecting chromosome loss in Hela cells and in mouse brain [10, 40]. In a similar fashion, monochromatic cells in tissues from dichromatic mice can be identified and isolated by FACS. In addition, it may be possible to detect monochromatic cells in situ in tissue sections. While other approaches provide data on mutation and mis-segregation events in tissues, only the dichromatic model can provide information about the locations of variant cells within tissues, and this information has the potential to reveal mitotic recombination events because such events can generate twin spots composed of neighboring patches of monochromatic cells of different colors descended from the monochromatic daughter cells produced from a mitotic cell that has undergone crossing over between homologous chromosomes .
Sources and features of expression noise
Mouse cells exhibited both extrinsic and intrinsic noise, the two types of variation seen in expression of allelic genes in yeast and bacteria [20, 21]. Extrinsic noise refers to variation in the fluorescence intensity emitted by a given fluorescent protein in different cells in the population [20, 21], and is thought to be due to variation among cells with respect to parameters such as position in the cell cycle. ES cells and MEFs exhibited similar levels of extrinsic noise, which may seem surprising given that MEF populations are derived from the numerous cell types present in a 13.5 dpc embryo, while ES cell populations contain a single cell type. However, many of the diverse cell types present in a dissociated embryo appear to fail to proliferate, and cells resembling fibroblasts quickly predominate in MEF cultures. Another factor that may work to minimize extrinsic noise caused by heterogeneity with respect to cell types is the robust activity of the ROSA26 promoter, which is driving transcription of the fluorescent protein genes. This promoter is known to function in a wide array of cell types [41–50].
Intrinsic noise refers to the discordance in the intensities of the two fluorescent proteins within a single cell, and is thought to be due to lack of coordination with respect to processes such as assembly of transcription complexes at allelic promoters [20, 21]. This lack of coordination is thought to result from stochastic variation caused by a scarcity of factors needed to accomplish gene expression. Intrinsic noise occurred in both types of mouse cells, and to similar extents. The level of intrinsic noise exhibited by mouse cells resembled that seen in E. coli when the promoter driving fluorescent gene transcription was semi-repressed by the lac repressor protein . Intrinsic noise in E. coli was reduced when repression was lifted and transcription rate was increased, leading to the suggestion that intrinsic noise is inversely related to transcription rate . However, studies in yeast showed that the relationship between intrinsic noise and transcription can be more complex .
LOH at ROSA26 produced monochromatic cells in populations of dichromatic mouse embryonic cells and was usually accompanied by retention of heterozygosity at a locus between ROSA26 and the centromere, suggesting that mitotic recombination was the major cause of ROSA26 LOH. Dichromatic mouse embryonic cells exhibited expression noise, a phenomenon previously described in bacteria and yeast carrying different fluorescent protein genes as allelic markers. Dichromatic mouse embryonic cells provide a novel system for studying genetic/karyotypic stability and factors influencing expression from allelic genes in cultured ES cells, and suggest that similar approaches will allow these phenomena to be studied in tissues.
The two lines of transgenic mice used to make embryonic cell lines were a gift from F. Costantini . One transgenic mouse line (R26R-EYFP) carried a gene encoding enhanced YFP at the ROSA26 locus. The other mouse line (R26R-ECFP) carried a gene encoding enhanced CFP at the ROSA26 locus. We crossed the two lines to produce mice with different fluorescent protein markers at the ROSA26 locus. These mice were of mixed genetic background that included alleles from three inbred strains, 129X1/SvJ, C57BL/6J and FVB/n. This situation was due to the following history of mouse production and maintenance. Strain 129X1/SvJ was the background into which the fluorescent protein genes were originally integrated into the mouse genome by gene targeting . The targeted 129X1/SvJ ES cells were injected into C57BL/6J blastocysts and chimeric mice were bred to C57BL/6J females to obtain transgenic mice that were 129X1/SvJ/C57BL/6J hybrids. The fraction of alleles from the 129X1/SvJ background was reduced from 50% because the mouse lines were maintained by crossing to C57BL/6J mice. The degree of this reduction can only be estimated because the number of crosses that had been performed since derivation of the original 129X1/SvJ/C57BL/6J hybrid transgenic lines was not available. The FVB/n background was introduced when the R26R-ECFP and R26R-EYFP mice were each crossed to a line of FVB/n mice that expresses the Cre recombinase during early embryonic development . This cross was necessary to remove a floxed transcriptional stop cassette situated at the beginning of each fluorescent protein gene. We screened the offspring of these crosses and found that some of the mice expressed fluorescent protein ubiquitously, and analysis (by PCR) of the transgenes in fluorescent mice showed that the stop cassette had been removed. These results were as expected based on previous reports . We selected one mouse exhibiting YFP fluorescence and one exhibiting CFP fluorescent and used them to establish two lines (R26YFP and R26CFP) by inbreeding. Hence, it was expected that most loci in the R26YFP and R26CFP lines would be occupied by alleles from either C57BL/6J or FVB/n, although alleles from strain 129X1/SvJ could be present at some loci.
Derivation, culture and treatment of cell lines
Mouse embryonic fibroblast (MEF) polyclonal cell lines were derived as described . Briefly, R26YFP and R26CFP mice were mated and at 13.5 dpc pregnant mice were sacrificed. Embryos were harvested and heart, liver and blood were removed and discarded. The remaining tissue was minced and pieces suspended in 1 ml 0.05% trypsin-EDTA (Invitrogen, Carlsbad, CA) and subjected to 12–18 hour digestion in at 4°C. Following digestion, cells were mechanically disaggregated by repeated pipetting in Dulbecco's modified eagle media (DMEM), supplemented with 10% FBS (Invitrogen, Carlsbad, CA) and 200 mM L-glutamine (Invitrogen, Carlsbad, CA) (complete DMEM). Cells from one embryo were divided among four 10 cm cell culture dishes and cultured overnight in DMEM, 200 mM L-glutamine, 10% FBS. These cells were trypsinized and cells divided among four plates. A day later, the cells were harvested and cryopreserved. For analysis, a vial of cells was thawed and placed in complete DMEM in a single 10 cm culture dish, which was incubated at 37°C until the plate was confluent (12 to 24 h).
To obtain ES cells, male R26YFP mice were crossed to female R26CFP mice. Derivation of ES cells was as described . Briefly, blastocysts were harvested at embryonic day 3.5 and put into culture in ES cell media (Dulbecco's modified eagle media (DMEM) that was supplemented with 15% certified FBS (Invitrogen, Carlsbad, CA), 200 mM L-glutamine (Invitrogen), 0.1 mM β-mercaptoethanol (Sigma, St. Louis, MO) and leukemia inhibitory factor (LIF), 1000 U/ml (Chemicon, Temecula, CA)). Two cultured blastocysts each yielded a cell line that maintained morphology characteristic of mouse ES cells.
ES cell lines were maintained on primary embryonic mouse fibroblast (MEF) feeder layers, mitotically inactivated by treatment with mitomycin C (Fisher, Pittsburgh, PA) 0.01 μg/ml in DMEM for 1–2 hours, using standard incubation conditions: 37°C, 10% CO2 .
ES cells were mutagenized by the procedure described by Munroe et al. . Briefly, approximately 1 million ES cells on a feeder layer were exposed to culture media containing 0.6 mg/ml ethylmethanesulfonate (EMS) for 20 hours, at which time culture media was removed, cells were washed several times and then placed in ES cell media and incubated at 37°C for 10 days. Cells were harvested and analyzed by FACS.
Cells were removed from plates by digestion with 0.25% trypsin-EDTA (Invitrogen, Carlsbad, CA) at 37°C for 5 to 15 min. Cells were suspended in sorting buffer (Ca++ & Mg++ free PBS, 1 mM EDTA, 25 mM Hepes,1%FBS), transferred into Falcon FACS tubes with cell strainers (35-2235)(BD Biosciences, San Jose, CA) and examined by light microscopy to verify the absence of aggregates.
Cells were sorted using a FACSVantage™ SE (BD Biosciences, San Jose, CA) flow cytometer equipped with digital DiVa Software, an argon laser (488 nm), Coherent Innova krypton laser tuned to 407 nm, 90 micron nozzle using a sheath pressure of 26 pounds per square inch, standard optics including a 530/30 nm band-pass filter (FL1) for YFP and 480/30 nm band-pass filter (FL4) for CFP. Daily alignment was performed using chicken red blood cells (BioSure, Grass Valley, CA) and AlignFlow Plus UV beads (Molecular Probes, Eugene, OR) for the 488 nm laser and UV laser, respectively.
Cells were first gated based on forward and side scatter. Then, cells were analyzed for both CFP and YFP fluorescence. Gates for isolation of monochromatic embryonic mouse cells were established using cells (either MEF or ES) made for the purpose. These cells carried and expressed either a YFP or a CFP gene. Sorted cells were collected into 1.5 ml conical tubes containing cell culture media, supplemented with 1× antibiotic-antimycotic (Invitrogen). Cells recovered from the FACS were cultured in media containing antibiotics.
ES cells subjected to FACS were harvested from plates that contained both transgenic ES cells and wild type MEF feeder cells. Although MEF feeder cells did not carry either fluorescent protein, it was expected that these cells might contribute to the FACS data due to autofluorescence. In fact, the FACS data produced by ES cell cultures contained a small number of faintly fluorescent points. To determine if these points were from feeder cells, the dim objects were isolated and placed in culture dishes. The next day, the dishes were inspected by microscopy, which showed the presence of adherent cells that were morphologically identical to feeders and were dimly fluorescent.
Data files were saved for every sort and subsequently analyzed using FlowJo software (TreeStar, San Carlos, CA). Frequencies reported are based on analysis of 2.5 × 106 events per sort. Statistical comparisons were performed using the Student's paired T-test.
Intrinsic and extrinsic expression noise values were calculated using formulas previously described [20, 21]. Calculations were performed using CFP fluorescence values that had been adjusted to correct for the intrinsic faintness of CFP molecules compared to YFP molecules. An adjustment factor was determined for each cell line by comparing the population mean YFP fluorescence to the population mean CFP fluorescence. This factor varied slightly from one cell line to the other, but, generally, CFP fluorescence values used in noise calculation were approximately 2 fold higher the values reported by the FACS.
Rates of allele loss were obtained by studying the frequency of monochromatic variants in populations that arose during the expansion of populations that initially lacked monochromatic variants, which were obtained by FACS. Rates were estimated by dividing the proportions of variants by the number of generations estimated to have elapsed during expansion of initially variant-free populations [30, 31].
Phenotypic analysis of R26CY subclones
FACS-isolated subclones were expanded in culture and then analyzed by fluorescent microscopy on a Nikon E400 microscope. The filter set for CFP provided excitation light wavelengths between 426 and 446 nm and allowed detection of emitted light between 460 and 500 nm. The filter set for YFP provided excitation light wavelengths between 490 and 510 nm and allowed detection of emitted light between 520 and 550 nm. Experiments with monochromatic cells showed that the signals from the two fluorescent proteins could be detected without interference of one with the other. Images were captured using a Spot Jr. CCD camera (Diagnostic Instruments, Sterling Heights, MI).
DNA analysis of R26CY subclones
ES cells were first separated from feeder cells by FACS. This separation was easily accomplished because feeder cells were not very fluorescent. DNA to be used for PCR was isolated from cells by proteinase K digestion in lysis buffer (5 mM EDTA, 200 mM NaCl, 100 mM Tris, 0.2% SDS) 8–12 hours at 57°C followed by isopropanol precipitation. PCR was performed in 50 μl containing 2.5 mM MgCl2 (Promega, Madison, WI), 1× PCR buffer (Promega), 1.6 mM dNTPs, 0.5 U Taq polymerase (Promega).
To analyze the ROSA26 locus, Primer 1 (5'CAGTAGTCCAGGGTTTCCTTGATG) which is specific to the ROSA26 promoter region, was paired with Primer 2 (5'GTCGCGGCCGCTTTACTTGT), which is common to both the CFP and YFP genes. Cycling conditions were as follows: 3 min denaturation at 94°C followed by 35 cycles of 94°C for 1 min; 58°C for 1 min, 72°C for 1 min, after which there was a final 5 min extension reaction at 72°C. The 1 Kb (approximate size) product was purified using the QIAquick PCR purification kit (Qiagen, Valencia, CA). CFP and YFP PCR products could be distinguished by restriction fragment length polymorphism (RFLP) analysis with Pst1 endonuclease (10 U/μl)(Invitrogen) using gel electrophoresis (1.5% agarose). The CFP product contains one Pst1 recognition site yielding bands of roughly 800 and 200 bp in size. The YFP product has two Pst1 sites and generates bands of approximately 500, 300 and 200 bp in size. To confirm the results of the Pst1 RFLP analysis, PCR products were cloned into TOPO-TA (Invitrogen) and sequenced. Statistical significance was tested using the binomial distribution.
LOH at ROSA26 might be accompanied by LOH at linked loci. To identify linked loci that would be informative, i.e. heterozygous in the parental ES cell lines R26CY1 and R26CY2, were sought. The first step was to identify candidate loci using the Mouse Genome Informatics (MGI) database . Several loci were then tested by PCR and sequencing performed by Polymorphic DNA technologies (Alameda, CA) . The D6MIT159 locus was found to be heterozygous. Genomic DNA samples were subjected to PCR using the primers described in the MGI database. PCR products were resolved using gel electrophoresis through a 3% agarose gel. Parental cells produced the two bands expected. Cells that had lost heterozygosity at D6MIT159 produced only one band. In all cases of LOH at D6MIT159, the band that was retained was the one linked to the ROSA26 allele retained.
Metaphase chromosome preparations for spectral karyotyping (SKy) analysis were prepared by treating 50–70% confluent cultures with 10 μg/ml colchicine (Sigma) for 2 hours. Following treatment, the cells were lifted with 0.25% trypsin, re-suspended in ES cell media, collected by centrifugation at 1300 RPM for 5 minutes, and re-suspended in 10 ml hypotonic solution (KCl 3 g/L, HEPES 4.8 g/L, EGTA 0.2 g/L, NaOH 0.36 g/L, pH7.4). This suspension was incubated at 37°C for 50 minutes. Subsequently, 2 ml of fixative (methanol acetic acid 3:1) was added, the cells were collected by centrifugation at 2000 RPM for 5 minutes and the pellet was re-suspended in 12 ml fixative. The cells were stored at -80°C until use. The SKy was performed by the SKy/FISH facility at the Roswell Park Cancer Institute (Buffalo, NY).
Whole chromosome painting was performed on metaphase chromosomes prepared as described above. Chromosome spreads were prepared as previously described . Chromosome paints for chromosome 6 (starfish paints) were purchased from Cambio Ltd. (Cambs, UK). Hybridizations were performed according to the manufacturer's protocol. Fluorescent images were collected on a Leica TCS SP2 confocal microscope and edited using Velocity software.
Estimation of the maximum number of trisomic cells in a population was obtained from the following relationship: (1-f)x = 1-p, where p is the probability of seeing at least 1 trisomic cell, x is the number of cells examined. and f is the frequency of trisomy in the population. When p is set at 0.95, and f at 0.05, X is 59. Thus, if one examines 59 cells and does not see a trisomic cell one can conclude with 95% confidence that the frequency of trisomic cells in the population is 5% or less.
The authors thank the Flow cytometry core at Children's Hospital, Cincinnati, the gene targeting and DNA sequencing cores of the University of Cincinnati, Yuri Nikiforov and Manoj Gandhi for assistance with FISH and confocal microscopy. Jon S. Larson was supported by the National Institute for Environmental Health Sciences (NIEHS) training grant T32 ES07250. This work was supported by grants P42 ES04908 and P30-ES06096 from the NIEHS.
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