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Table 3 Oligos

From: Recombination Phenotypes of Escherichia coli greA Mutants

aof2

TCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTTAGGTGGCGGTACTTGGGT

ARB1a

GGCCACGCGTCGACTAGTACNNNNNNNNNNGATAT

ARB2a

GGCCACGCGTCGACTAGTACNNNNNNNNNNACGCC

ARB6a

GGCCACGCGTCGACTAGTAC

bpa

TCTGGTGGCCGGAAGGCGAAGCGGCATGCATTTACGTTGAGGTCTGACAGTTACCAATGC

BLZ3

GAACTTTAAAAGTGCTCATCATTG

BLZ4

GGGTTTTCCCAGTCACG

cat27

TCGGAGATCCCCCGGGCTGCAGGAATTCGATATCAAGCTTATGAGACGTTGATCGGCACG

cat28

AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTCAGGCGTAGCACCAGGC

cat29

GACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGGATCCATGAGACGTTGATCGGCACG

cat30

TGGCTAAATACGGAAGGATCTGAGGTTCTTATGGCTCTTGGATCCATTCAGGCGTAGCACCAGGC

cat34

TCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGAATGAGACGTTGATCGGCACG

cat35

GCGTACTGTGACTTCTTCTGCCGGGATCTTCACGCTCTCAATTCAGGCGTAGCACCAGGC

cat38

AGGTTGATATTGATTCAGAGGTATAAAACGAATGAGTACTATGAGACGTTGATCGGCACG

cat39

CCTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGATTCAGGCGTAGCACCAGGC

cat40

CACCGTTACACATATGCAGGATGAAGCAGCCAACTTCCCGATGAGACGTTGATCGGCACG

cat41

CGCAGTTCGAGGCTGAACTCTTCTTCCTGGGCTGCACGGTATTCAGGCGTAGCACCAGGC

D41A

AGCTGCGTGGTATTCGGCGTTTTCTTTCAGGGCGCCATGCTCACGCGCTTCCGCGATAGCAGCAA

D41N

AGCTGCGTGGTATTCGGCGTTTTCTTTCAGGTTGCCATGCTCACGCGCTTCCGCGATAGCAGCAA

dksAD

AGTGCGTGTTAAGGAGAAGCAACATGCAAGAAATGGCTGGCTAATTACAGCCGTTCCATCACGT

dksNN

CATATGCAGGATGAAGCAGCCAACTTCCCGAACCCGGTAAACCGTGCAGCCCAGGAAGAAGAGTTCAGCC

E44K

CTGTTCACGAGCTGCGTGGTATTCGGCGTTTTTTTTCAGGTCGCCATGCTCACGCGCTTCCGCGA

greA5

CATCATCATGAATTCAGCTGGTCAATCCGATGA

greA6

ACTACTACTGGATCCTGTACAGGTCATCGGTAAAACG

greA9

TGAAATCATTGCTGCTATCGCGGAAGCGCGTGAGCATGGCCTCGACATCTTGGTTACCGT

greAD

ACATCTTGAGTATTGGGTAATTCTTACAGGTATTCAGCTTGCATTTGTTATACCTCTTGAATATTCCTGA

greAX

CTGTTCACGAGCTGCGTGGTATTCGGCGTTTTCTTTCAGGCGCGGAATAACATCATTTGG

greAY

GCTGCTATCGCGGAAGCGCGTGAGCATGGCGACCTGAAAGCTCGACATCTTGGTTACCGT

greAZ

AGAAACCCTGCTGTTCACGAGCTGCGTGGTATTCGGCGTTCGCGGAATAACATCATTTGG

PRO1

TGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTACAACCAATTAACCAATTCTG

PRO2

GTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCACGTTGTGTCTCAAAATCTC

Rtet1a

CCACTTAATGTGATCATTGAACC

Rtet2a

AACCATTTTCAGTGATCCATTGC

SP01

ATTCCTGAAACAGAAAGCCGCAGAGCAGAAGGTGGCAGCATCCATGGAAAAGAGAAG

SP02

TTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGCATATGAATATCCTCCTTAG

SR2

GACTACGACTACATCTAGATTCGAACATCGATATGGGCAACTCTATC

SR3

ATCAGCCATCATGGATCCGTTTGCAATGGCGTACCTT

  1. a. Arbitrary PCR and sequencing to locate transposon insertions was done as described [47], but with primers designed for Tn10d(tet). The first round of PCR was done with primers Rtet1, ARB1, and ARB6; the second with Rtet2 and ARB2. Sequencing was done with Rtet2.
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BMC Molecular Biology

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