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  1. Methodology article

    Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection

    Targeted genomic editing using the CRISPR/Cas9 methodology has opened exciting new avenues in probing gene function in virtually any model system, including cultured mammalian cells. Depending on the desired m...

    Jason A. Estep, Erin L. Sternburg, Gissell A. Sanchez and Fedor V. Karginov

    BMC Molecular Biology 2016 17:9

    Published on: 2 April 2016

  2. Methodology article

    A reliable method for quantification of splice variants using RT-qPCR

    The majority of protein isoforms arise from alternative splicing of the encoding primary RNA transcripts. To understand the significance of single splicing events, reliable techniques are needed to determine t...

    Julia Camacho Londoño and Stephan E. Philipp

    BMC Molecular Biology 2016 17:8

    Published on: 15 March 2016

  3. Research article

    Gene expression profiling of the venom gland from the Venezuelan mapanare (Bothrops colombiensis) using expressed sequence tags (ESTs)

    Bothrops colombiensis is a highly dangerous pit viper and responsible for over 70 % of snakebites in Venezuela. Although the composition in B. colombiensis venom has been identified us...

    Montamas Suntravat, Néstor L. Uzcategui, Chairat Atphaisit, Thomas J. Helmke, Sara E. Lucena, Elda E. Sánchez and A. Rodríguez Acosta

    BMC Molecular Biology 2016 17:7

    Published on: 5 March 2016

    The Erratum to this article has been published in BMC Molecular Biology 2016 17:13

  4. Research article

    Knockdown of SALL4 inhibits the proliferation and reverses the resistance of MCF-7/ADR cells to doxorubicin hydrochloride

    Breast cancer is the most frequent malignancy in women and drug resistance is the major obstacle for its successful chemotherapy. In the present study, we analyzed the involvement of an oncofetal gene, sal-lik...

    Yuan-Yuan Chen, Zhi-Zhen Li, Yuan-Yuan Ye, Feng Xu, Rui-Jie Niu, Hong-Chen Zhang, Yi-Jian Zhang, Ying-Bin Liu and Bao-San Han

    BMC Molecular Biology 2016 17:6

    Published on: 2 March 2016

  5. Reviewer acknowledgement

    Annual acknowledgement of reviewers

    The editors of BMC Molecular Biology would like to thank all our reviewers who have contributed to the journal in Volume 16 (2015).

    Timothy R. Sands

    BMC Molecular Biology 2016 17:5

    Published on: 19 February 2016

  6. Research article

    Structural and functional analysis of four non-coding Y RNAs from Chinese hamster cells: identification, molecular dynamics simulations and DNA replication initiation assays

    The genes coding for Y RNAs are evolutionarily conserved in vertebrates. These non-coding RNAs are essential for the initiation of chromosomal DNA replication in vertebrate cells. However thus far, no informat...

    Quirino Alves de Lima Neto, Francisco Ferreira Duarte Junior, Paulo Sérgio Alves Bueno, Flavio Augusto Vicente Seixas, Madzia Pauline Kowalski, Eyemen Kheir, Torsten Krude and Maria Aparecida Fernandez

    BMC Molecular Biology 2016 17:1

    Published on: 5 January 2016

  7. Research article

    Transfection of Sertoli cells with androgen receptor alters gene expression without androgen stimulation

    Androgens play an important role for the development of male fertility and gained interest as growth and survival factors for certain types of cancer. Androgens act via the androgen receptor (AR/Ar), which is inv...

    D. Fietz, M. Markmann, D. Lang, L. Konrad, J. Geyer, S. Kliesch, T. Chakraborty, H. Hossain and M. Bergmann

    BMC Molecular Biology 2015 16:23

    Published on: 29 December 2015

  8. Research article

    Conserved and highly expressed tRNA derived fragments in zebrafish

    Small non-coding RNAs (sncRNAs) are a class of transcripts implicated in several eukaryotic regulatory mechanisms, namely gene silencing and chromatin regulation. Despite significant progress in their identifi...

    Ana Raquel Soares, Noémia Fernandes, Marisa Reverendo, Hugo Rafael Araújo, José Luís Oliveira, Gabriela M. R. Moura and Manuel A. S. Santos

    BMC Molecular Biology 2015 16:22

    Published on: 22 December 2015

  9. Research article

    Temperature-induced variation in gene expression burst size in metazoan cells

    Gene expression is an inherently stochastic process, owing to its dynamic molecular nature. Protein amount distributions, which can be acquired by cytometry using a reporter gene, can inform about the mechanis...

    Ophélie Arnaud, Sam Meyer, Elodie Vallin, Guillaume Beslon and Olivier Gandrillon

    BMC Molecular Biology 2015 16:20

    Published on: 25 November 2015

    The Erratum to this article has been published in BMC Molecular Biology 2016 17:2

  10. Research article

    DNA double-strand break repair is impaired in presenescent Syrian hamster fibroblasts

    Studies of DNA damage response are critical for the comprehensive understanding of age-related changes in cells, tissues and organisms. Syrian hamster cells halt proliferation and become presenescent after sev...

    Ljudmila Solovjeva, Denis Firsanov, Anastasia Vasilishina, Vadim Chagin, Nadezhda Pleskach, Andrey Kropotov and Maria Svetlova

    BMC Molecular Biology 2015 16:18

    Published on: 12 October 2015

  11. Research article

    Deciphering targeting rules of splicing modulator compounds: case of TG003

    Recent advances in the development of small chemical compounds that can modulate RNA splicing brought excitement to the field of splicing-targeting therapy. Splicing-targeting therapy tries to ameliorate the d...

    Maki Sakuma, Kei Iida and Masatoshi Hagiwara

    BMC Molecular Biology 2015 16:16

    Published on: 24 September 2015

  12. Review

    TRIM28 as a novel transcriptional elongation factor

    TRIM28 is a multidomain protein with versatile functions in transcription and DNA repair. Recently it was shown that this factor plays unanticipated roles in transcriptional elongation. TRIM28 was shown to sta...

    Heeyoun Bunch and Stuart K Calderwood

    BMC Molecular Biology 2015 16:14

    Published on: 21 August 2015

  13. Research article

    Analysis artefacts of the INS-IGF2 fusion transcript

    In gene expression analysis, overlapping genes, splice variants, and fusion transcripts are potential sources of data analysis artefacts, depending on how the observed intensity is assigned to one, or more gen...

    Rasmus Wernersson, Thomas Frogne, Claude Rescan, Lena Hansson, Christine Bruun, Mads Grønborg, Jan Nygaard Jensen and Ole Dragsbæk Madsen

    BMC Molecular Biology 2015 16:13

    Published on: 29 July 2015

  14. Research article

    SIRT6 protein deacetylase interacts with MYH DNA glycosylase, APE1 endonuclease, and Rad9–Rad1–Hus1 checkpoint clamp

    SIRT6, a member of the NAD+-dependent histone/protein deacetylase family, regulates genomic stability, metabolism, and lifespan. MYH glycosylase and APE1 are two base excision repair (BER) enzymes involved in mut...

    Bor-Jang Hwang, Jin Jin, Ying Gao, Guoli Shi, Amrita Madabushi, Austin Yan, Xin Guan, Michal Zalzman, Satoshi Nakajima, Li Lan and A-Lien Lu

    BMC Molecular Biology 2015 16:12

    Published on: 11 June 2015

  15. Research article

    MicroRNA-19a regulates lipopolysaccharide-induced endothelial cell apoptosis through modulation of apoptosis signal-regulating kinase 1 expression

    MicroRNAs, small non-encoding RNAs that post-transcriptionally modulate expression of their target genes, have been implicated as critical regulatory molecules in endothelial cells.

    Wei-Long Jiang, Yu-Feng Zhang, Qing-Qing Xia, Jian Zhu, Xin Yu, Tao Fan and Feng Wang

    BMC Molecular Biology 2015 16:11

    Published on: 16 May 2015

  16. Reviewer acknowledgement

    Annual acknowledgement of reviewers

    The editors of BMC Molecular Biology would like to thank all our reviewers who have contributed to the journal in Volume 15 (2014).

    Tim Sands

    BMC Molecular Biology 2015 16:10

    Published on: 14 May 2015

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