Fig. 1
From: RNA polymerase II depletion promotes transcription of alternative mRNA species
Polymerase stress increases transcription of the long RPB2 mRNA. a Northern analysis [29] of mRNA levels when shifting the rpb1-1 mutant to the non-permissive temperature of 37 °C. Northern blot analysis of mRNA levels in the b RNAPII anchor-away cells (RPB1-FRB; MVY859) and c Tbp1 anchor-away cells (TBP1-FRB; MVY868) after addition of rapamycin to deplete FRB tagged proteins from the nucleus. The long and short isoform of the RPB2 mRNA are indicated