Fig. 5

miR-150 directly targets the 3′UTR of FOXO4. a The expression of FOXO4 in three C-33A sub-cell lines was determined by western blot. b The sequence of the wild type and mutant 3′ UTR of FOXO4 (mut-3′UTR) for luciferase reporter assay are shown. c Three C-33A sub-cell lines were transfected with reporter plasmids containing wild type or mutant 3′UTR of FOXO4 for 48 h and the luciferase activity was measured. Mean values ± SD for 3 independent experiments is shown. d Hypothetic illustration of how miR-150 promotes cervical cancer cell cycle progression and survival by targeting FOXO4