Figure 4

Zeb1 induces Ets1 expression, and it mediates much of the effect of Rb1 on Ets1 expression. (A) Zeb1 mutation leads to a gene dosage-dependent downregulation of Ets1 expression. Real time PCR is shown and results are normalizaed to β-actin mRNA when the Western blots show similar result accordingly. (B) Zeb1 mutation leads to a gene dosage-dependent induction of miR-200 family members. Real time PCR is shown. (C) Rb1 family mutation leads to induction of miR-200 family members, and this induction is reversed by lentiviral knockdown of Zeb1 (Zeb1 sh). (D) Zeb1 is induced along with Ets1 as Rb1 family members are mutated. And, knockdown of Zeb1 reverses most of the induction of Ets1. Real time PCR is shown.