Figure 2From: Efficient isolation of specific genomic regions retaining molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteinsExpression and purification of r3xFNLDD-D. (A) Coomassie Brilliant Blue (CBB) staining of the recombinant protein. The purified proteins were subjected to SDS-PAGE and CBB staining. (B) Immunoblot analysis (IB) of r3xFNLDD-D. The purified proteins were subjected to SDS-PAGE and IB with anti-Dock Ab. Supernatant: the supernatant prepared from the silkworm pupal homogenates. Precipitant: the insoluble precipitate prepared from the silkworm pupal homogenates. Elution: the eluate after affinity purification with Dock Catch Resin.Back to article page