Figure 3

Analysis of upstream motif promoter activity. The panels show the effects of additional upstream sequence length, and of mutation of sub-motifs, on the transcriptional activity of each of the three promoters PSE/UM1, UM2 and UM3, respectively. (A) UM1 from the SL2 RNA locus CeN7. "PSEA" and "PSEB" are the most invariant sequence elements in the PSE/UM1. (B) UM2 from the snoRNA locus CeN37. "BoxA" and "BoxB" show the two sub-motifs in UM2. (C) UM3 from the sbRNA locus CeN74-2. "PSEB" and "GTATA" are the most invariant sequence elements of the UM3. "Gene start" corresponds to the first 69, 32 and 56 bp of the CeN7, CeN74-2 and CeN37 mature transcripts, respectively. Relative expression levels are calculated by normalizing the qRT-PCR intensities of each CeNX_100 construct to 100. Sub-motif mutations were performed on the constructs containing the longest upstream sequences (e.g. CeN7_1k) mainly by converting each purine and pyrimidine residue to the opposite purine and pyrimidine, respectively (i.e. A to G, and vice versa).