Skip to main content

Table 3 Standard curve parameters for the candidate reference genes

From: Expression profiles of the pluripotency marker gene POU5F1 and validation of reference genes in rabbit oocytes and preimplantation stage embryos

Genes

Slope (m)

Intercept (b)

Efficiency (E)

Correlation (R2)

Actb

-3.37

21.90

98.0

97.9

Gapdh

-3.43

18.58

97.7

99.4

Hprt1

-3.25

22.56

103.1

98.9

H2afz

-3.52

18.85

92.3

99.4

Ppia

-3.51

16.07

92.6

99.7

Eef1e1

-3.48

20.50

94.0

97.2

G6pdx

-3.09

23.69

110.7

99.2

Polr2a

-3.38

22.10

97.7

96.3

Pgk1

-3.51

16.89

92.8

99.4

Ywhaz

-3.42

20.09

96.0

98.9

POU5F1

-3.83

17.32

82.4

99.8