Genomic actions of 1,25-dihydroxyvitamin D3 on insulin receptor gene expression, insulin receptor number and insulin activity in the kidney, liver and adipose tissue of streptozotocin-induced diabetic rats

Table 2 Total DNA, RNA and protein content in different tissues from the four groups of rats

	Control	1,25D ₃	STZ	STZ + 1,25D ₃
Kidney
DNA (mg/g)	3.0 ± 0.1	2.8 ± 0.2	3.3 ± 0.5	3.4 ± 0.1^ab
Protein(mg/g)	69 ± 2	120 ± 5^a	134 ± 33^a	153 ± 29^ab
Prot/DNA (mg/mg)	18 ± 5	43 ± 2^a	41 ± 10^a	45 ± 10^a
RNA (mg/g)	1.3 ± 0.1	1.6 ± 0.2	1.3 ± 0.3	1.7 ± 0.3
Liver
DNA (mg/g)	1.9 ± 0.2	1.5 ± 0.2	2.9 ± 0.6^ab	2.4 ± 0.2^ab
Protein(mg/g)	105 ± 19	146 ± 17^a	203 ± 27^ab	182 ± 15^a
Prot/DNA (mg/mg)	52 ± 9	99 ± 11^a	67 ± 5	76 ± 3^a
RNA (mg/g)	3.9 ± 0.3	3.2 ± 0.4	3.5 ± 0.4	3.8 ± 0.2
Adipose tissue
DNA (mg/g)	0.2 ± 0.06	0.2 ± 0.02	0.3 ± 0.03	0.3 ± 0.04
Protein(mg/g)	5.1 ± 1	8.2 ± 0.8^a	7.9 ± 0.5^a	6.1 ± 1
Prot/DNA (mg/mg)	24 ± 5	47 ± 6^a	25 ± 1^b	20 ± 3
RNA (mg/g)	0.1 ± 0.02	0.1 ± 0.01	0.1 ± 0.03	0.1 ± 0.04

Sham-treated rats (Control), rats treated with 1,25D₃ [150 IU/Kg (3.75 μg/Kg) one a day, for 15 days] (1,25D₃), streptozotocin-induced diabetic rats (STZ) and streptozotocin-induced diabetic rats treated with 1,25D₃ (STZ+1,25D₃).
Values are the mean ± SEM of 4–6 determinations in each group.
^a p < 0.05 vs. Control-rats; ^b p < 0.05 vs. 1,25D₃-rats; ^c p < 0.05 vs. STZ-rats.

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