Table 1 20 most significantly deregulated genes after transfection of GFP siRNA.
downregulated | HELA | HEK | |||||||
|---|---|---|---|---|---|---|---|---|---|
# | name | RefSeq | molecular function | MX | p§ | BS | MX | p§ | BS |
1 | SOAT1 | NM_003101 | sterol O-acyltransferase activity | -2.28 | 8.72E-14 | 32.76 | -0.83 | 8.18E-07 | 14.15 |
2 | Q96HQ8 | ~ | unknown | -1.95 | 8.72E-14 | 32.87 | -1.52 | 7.71E-12 | 27.43 |
3 | CASP8 | NM_033356 | signal transducer activity;caspase activity | -1.56 | 5.35E-13 | 30.32 | -0.88 | 6.42E-09 | 20.37 |
4 | C10orf56 | NM_153367 | nucleic acid binding;zinc ion binding | -1.62 | 6.03E-13 | 30.12 | -0.49 | 1.35E-05 | 10.51 |
5 | NUDT3 | NM_006703 | magnesium ion binding;hydrolase activity | -1.45 | 1.74E-12 | 28.78 | -0.42 | 6.64E-05 | 8.35 |
6 | RAB21 | NM_014999 | nucleotide binding;GTP binding | -1.55 | 1.89E-12 | 28.60 | -0.76 | 1.51E-07 | 16.52 |
7 | HMGCS1 | NM_002130 | hydroxymethylglutaryl-CoA synthase activity | -1.48 | 2.73E-12 | 28.09 | -0.45 | 6.05E-05 | 8.47 |
8 | PAICS | NM_006452 | catalytic activity;ATP binding | -1.37 | 3.72E-12 | 27.62 | -0.66 | 3.61E-07 | 15.39 |
9 | TM16F | NM_001025356 | integral to membrane | -1.59 | 4.96E-12 | 27.25 | -0.45 | 2.18E-04 | 6.68 |
10 | GOSR1 | NM_004871 | v-SNARE activity | -1.47 | 5.13E-12 | 27.13 | -0.46 | 6.89E-05 | 8.29 |
11 | PSF1 | NM_021067.3 | protein binding | -2.18 | 5.44E-12 | 27.01 | -1.55 | 5.47E-09 | 20.81 |
12 | CYLD | NM_015247 | NFkB pathway | -1.39 | 6.75E-12 | 26.74 | -0.60 | 2.91E-06 | 12.56 |
13 | CXorf34 | NM_024917 | methyltransferase activity | -1.68 | 1.11E-11 | 26.08 | -0.67 | 1.08E-05 | 10.82 |
14 | SKP2 | NM_005983 | protein binding | -1.18 | 1.80E-11 | 25.33 | -0.74 | 7.49E-08 | 17.28 |
15 | IL6ST | NM_002184 | interleukin-6 receptor activity;protein binding | -1.20 | 2.92E-11 | 24.72 | -0.57 | 3.49E-06 | 12.27 |
16 | C10orf119 | NM_024834 | hypothetical protein | -1.14 | 3.13E-11 | 24.63 | -0.26 | 5.18E-03 | 2.29 |
17 | LARS2 | NM_015340 | leucine-tRNA ligase activity;ATP binding | -1.18 | 5.29E-11 | 23.85 | -0.49 | 2.65E-05 | 9.59 |
18 | NR1D2 | NM_005126 | transcription factor activity | -1.12 | 5.63E-11 | 23.71 | -0.32 | 1.36E-03 | 4.11 |
19 | ACSL4 | NM_022977 | long-chain-fatty-acid-CoA ligase activity | -1.22 | 6.44E-11 | 23.51 | -0.47 | 7.21E-05 | 8.21 |
20 | CCD25 | NP_060716 | unknown | -1.09 | 1.72E-10 | 22.18 | -0.33 | 1.67E-03 | 3.83 |
upregulated | HELA | HEK | |||||||
# | name | RefSeq | molecular function | M X | p § | B S | M X | p § | B S |
1 | ANXA3 | NM_005139 | diphosphoinositol-polyphosphate phosphatase | 2.75 | 1.13E-15 | 37.54 | 0.47 | 3.89E-05 | 9.08 |
2 | LOC51149 | NM_016175 | hypothetical protein | 1.29 | 3.48E-11 | 24.46 | 0.29 | 8.79E-03 | 1.61 |
3 | DDAH1 | NM_012137 | hydrolase activity | 1.02 | 1.87E-10 | 22.07 | 0.35 | 5.88E-04 | 5.27 |
4 | UBP18 | NM_017414.2 | ubiquitin thiolesterase activity | 1.00 | 1.99E-10 | 21.99 | 0.54 | 4.64E-06 | 11.91 |
5 | IL15RA | NM_002189 | interleukin receptor activity | 1.01 | 2.06E-10 | 21.94 | 0.30 | 2.38E-03 | 3.34 |
6 | SPHK1 | NM_021972 | calmodulin binding;transferase activity | 1.01 | 2.16E-10 | 21.88 | 0.42 | 9.44E-05 | 7.85 |
7 | HIST1H2AC | NM_003512.3 | DNA binding | 1.27 | 2.49E-10 | 21.68 | 0.54 | 7.94E-05 | 8.08 |
8 | MAG1 | NM_032717 | acyltransferase activity | 0.95 | 2.70E-10 | 21.53 | 0.40 | 1.21E-04 | 7.53 |
9 | SAT1 | NM_002970 | acyltransferase activity | 1.08 | 3.21E-10 | 21.24 | 0.45 | 1.46E-04 | 7.25 |
10 | PTGS2 | NM_000963 | peroxidase activity;oxidoreductase activity | 1.25 | 4.65E-10 | 20.78 | 0.38 | 3.44E-03 | 2.84 |
11 | HDHD1A | NM_012080 | catalytic activity | 0.95 | 8.31E-10 | 20.03 | 0.35 | 8.84E-04 | 4.67 |
12 | STAMBPL1 | NM_020799 | ubiquitin thiolesterase activity | 0.80 | 9.32E-10 | 19.90 | 0.30 | 8.23E-04 | 4.78 |
13 | PIGL | NM_004278 | hydrolase activity | 0.83 | 1.17E-09 | 19.61 | 0.36 | 2.10E-04 | 6.73 |
14 | CLPB | NM_030813 | nucleoside-triphosphatase activity | 0.81 | 2.28E-09 | 18.74 | 0.28 | 3.67E-03 | 2.75 |
15 | SMPDL3B | NM_014474 | hydrolase activity, acting on glycosyl bonds | 0.88 | 2.40E-09 | 18.67 | 0.33 | 1.45E-03 | 4.02 |
16 | HERC6 | NM_017912 | ubiquitin-protein ligase activity;GEF activity | 0.81 | 2.69E-09 | 18.52 | 0.43 | 5.32E-05 | 8.62 |
17 | GLRB | NM_000824 | inhibitory glycine receptor,CNS | 0.79 | 4.31E-09 | 17.90 | 0.28 | 3.47E-03 | 2.82 |
18 | HHLA3 | NM_007071 | unknown | 0.73 | 5.02E-09 | 17.71 | 0.36 | 1.86E-04 | 6.89 |
19 | GNPDA1 | NM_005471 | hydrolase activity | 0.63 | 2.46E-08 | 15.68 | 0.36 | 1.54E-04 | 7.17 |
20 | PLEKHA7 | NM_175058 | unknown | 0.63 | 3.29E-08 | 15.32 | 0.28 | 1.97E-03 | 3.59 |