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Table 5 Statistical output from BestKeeper analysis.

From: Exercise induced stress in horses: Selection of the most stable reference genes for quantitative RT-PCR normalization

 

ACTB

B2M

GAPDH

HPRT

R18S

RPL32

SDHA

TFRC

UBB

N

30

30

30

30

30

30

30

30

30

G Mean [Ct]

17.85

19.84

23.20

25.34

9.07

21.15

24.51

26.54

19.25

A Mean [Ct]

17.86

19.85

23.22

25.35

9.10

21.15

24.52

26.56

19.26

Min [Ct]

16.92

18.55

20.99

24.11

7.97

20.10

23.22

25.11

17.94

Max [Ct]

19.23

21.86

25.05

27.76

12.02

22.42

26.91

28.88

21.46

SD [± Ct]

0.49

0.51

0.71

0.62

0.57

0.46

0.66

0.73

0.64

CV [% Ct]

2.77

2.57

3.08

2.43

6.28

2.18

2.70

2.75

3.35

coeff. of corr. [r]

0.890

0.921

0.708

0.963*

0.949*

0.832

0.983*

0.844

0.935*

p-value

0.001

0.001

0.001

0.001

0.001

0.001

0.001

0.001

0.001

  1. Genes are listed in alphabetical order. N: number of samples; G Mean [Ct]: geometric mean of the Ct; A Mean [Ct]: arithmetic mean of the Ct; Min and Max [Ct]: extreme values of the Ct; SD [Ct]: standard deviation of the Ct; CV [% Ct]: coefficient of variance expressed as percentage on the Ct level. * Indicates the best correlation between control genes and the BestKeeper index.
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BMC Molecular Biology

ISSN: 1471-2199

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