Gene | Primers | Primer sequences 5'-3' | PCR conditions | ||
---|---|---|---|---|---|
 |  |  | Denaturation | Annealing | Extension |
Falcipain 1 | Fal1RACE GSP1R | CAAGATAGGATAGAAGACTTCCTC | Â | Â | Â |
 | Fal1RACE GSP2R | CATCTTCTTCATTGGAGGATTC | 94°C 1' | 52°C 1.30' | 68°C 1.30' |
 | Fal1RACE GSP3R | CCATGTTGTAATCCATGGTCTTCAAC | 94°C 1' | 50°C 1.30' | 68°C 1.30' |
Falcipain 2 | Fal2RACE GSP1R | TTACCTTAATGGAATGAATGCATC | Â | Â | Â |
 | Fal2RACE GSP2R | TAGGAAAATAAAATAAAACAAAACCAA | 94°C 1' | 52°C 1' | 68°C 1.30' |
 | Fal2RACE GSP3R | CATGTGGTAATCCATGGTTAAAA | 94°C 1' | 52°C 1.30' | 68°C 1.30' |
Falcipain 2a | Fal2aRACE GSP1R | TTACCTTAATGGAATGAATGCATC | Â | Â | Â |
 | Fal2aRACE GSP2R | TAGGAAAATAAAATAAAACAAAACCAA | 94°C 1' | 52°C 1' | 68°C 1.30' |
 | Fal2aRACE GSP3R | TATGAATATACATATACACTAGG | 94°C 1' | 50°C 1.30' | 68°C 1.30' |
Falcipain 3 | Fal3RACE GSP1R | TTCAAGTAATGGTACATAAGCTTC | Â | Â | Â |
 | Fal3RACE GSP2R | CCTAGGATCGTTTATATTATTTGATAA | 94°C 1' | 52°C 1' | 68°C 1.30' |
 | Fal3RACE GSP3R | CATATGATATTCCATGGTTCAAAC | 94°C 1' | 50°C 1.30' | 68°C 1.30' |