Figure 2

Mapping of falcipains transcription start site. (A) Schematic representation of the non-coding region of the falcipains showing the transcription and translation start sites. Positions downstream of the ATG are denoted with a (+) sign and those upstream with a (-) sign. Thick lines denote regions before the transcription start site. Thin lines denote regions after the start site. Arrows denote the primers and their positions used for 5'RACE. (B) Agaorse gel electrophoresis of 5'RACE products. Lane M, DNA size markers. Arrows denotes the PCR amplified products.