Identification of valid reference genes for the normalization of RT qPCR gene expression data in human brain tissue

Table 4 Main clinical and neuropathological data for subjects

Diagnosis	Sex	Age	Braak Stage	PM Delay (min)	CSF pH
AD	f	81	4	193	6.54
	f	85	4	220	6.59
	f	77	5	215	6.67
	m	95	5	185	6.4
	m	58	6	385	6.42
	f	87	6	300	6.66
Mean ± S.D.		80.50 ± 11.49		249.67 ± 71.09	6.55 ± 0.11
PD	m	75	n/a	255	6.42
	f	83	3	290	6.76
	f	80	3	480	6.85
	m	82	3	425	7.64
	f	80	3	255	6.50
	f	84	1	370	6.50
Mean ± S.D.		80.67 ± 2.92		345.83 ± 86.09	6.78 ± 0.41
DLB	f	79	1	200	6.4
	m	74	1	255	6.12
	m	81	2	345	6.63
	m	86	2	472	n/a
	f	79	3	235	6.72
	m	95	2	270	6.6
Mean ± S.D.		82.33 ± 6.67		296.17 ± 90.07	6.49 ± 0.21
Controls	f	69	1	375	6.59
	f	72	1	405	6.52
	m	79	1	320	6.72
	m	83	1	275	6.49
	f	88	2	340	n/a
Mean ± S.D.		78.20 ± 6.97		343.00 ± 44.79	6.58 ± 0.09

Unpaired t-tests were used to verify that the AD, PD, DLB and control groups were matched for age, post-mortem (PM) delay and pH of CSF.

ISSN: 1471-2199