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Figure 4 | BMC Molecular Biology

Figure 4

From: The Mre11 protein interacts with both Rad50 and the HerA bipolar helicase and is recruited to DNA following gamma irradiation in the archaeon Sulfolobus acidocaldarius

Figure 4

Co-immunoprecipitation of Rad50, Mre11 and HerA by anti-Rad50 antibodies. (a) Western blot analysis of immuno-precipitates performed on extracts from exponentially growing S. acidocaldarius cells: tot, total extract; pel, chromosomal-enriched fraction; sup, soluble fraction; pDNase, chromosomal-enriched fraction previously treated with DNase I. (b) Western blot analysis of immuno-precipitates performed on a mixture of Rad50, Mre11, HerA and NurA recombinant proteins. C: Western blot control on (a) S. acidocaldarius total extracts and (b) recombinant proteins mixtures. preI: immunoprecipitations with pre-immune antibodies.

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BMC Molecular Biology

ISSN: 1471-2199

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