Figure 5
From: Iron-dependent degradation of IRP2 requires its C-terminal region and IRP structural integrity
Truncation of the C-terminal domain 4 stabilizes IRP2 against iron. (A) Schematic representation of the deletion mutants showing domains 1–4, the hinge which links domains 3 and 4, and the C-terminal HA tag. (B-F) H1299 cells engineered to express the above mutants (in two or three independent clones) were treated overnight (14 h) with 30 μg/ml FAC and lysates were subjected to Western blotting with HA (top) and β-actin (bottom) antibodies. The different migration of wild type IRP2 and the ΔC60 and ΔC168 deletion mutants is illustrated in (B).