Figure 2From: Analysis of the transcriptional activity of endogenous NFAT5 in primary cells using transgenic NFAT-luciferase reporter miceUnresponsiveness of the 9xNFAT-Luc reporter to hypertonicity in NFAT5-deficient cells. A) NFAT5+/+ and NFAT5-/- MEF were transfected with the 9xNFAT-Luc reporter and stimulated with PMA plus ionomycin or hypertonicity (460 mOsm/kg), with or without FK506, during 24 hours. Mean ± S.D of three independent experiments is shown. B) NFAT5-/- MEF were transfected with the 9xNFAT-Luc reporter and an empty vector (pBSK(+)) or an NFAT5 expression vector. Cells were stimulated with PMA plus ionomycin or hypertonicity (460 mOsm/kg), with or without FK506, during 24 hours. Mean ± S.E.M of three independent experiments is shown.Back to article page