Double-stranded oligonucleotides used in gel shift assays and mutagenesysa | ||
---|---|---|
P1/USFmer | 5'-GGCGGCCCACGTG TCCACCT-3' | |
USFmer b | 5'-GGCCAGACCACGTG GTCTGTTC-3' | |
Sp1mer b | 5'-CCCTTGGTGGGGGCGGGGCCTAAGCTGCG-3' | |
MyoDmer c | 5'-CCCCCCAACACCTGCTGCCTGA-3' | |
P1/USFmut | 5'-CCCACGGGCGGCCACA GTGTCCACCTATTC-3' | |
P1/Spmut | 5'-GTGAGCATA GT CGTA GAGCAT GGT AGA GTACCGC-3' | |
Q-PCR Primers | Amplified region | Sequences |
P1 ChIP f | P1 promoter AβH-J-J | 5'-AACGAACTAAATTCCTGCTTCAGC-3' |
P1 ChIP r | (contains USF elements) | 5'-TCCTTTGGAACGTGGCAAACGT-3' |
Neg ChIP f | negative control region | 5'-TGTGTGATTTCCCGTCAACTGTC-3' |
Neg ChIP r | (without USF elements) | 5'-CCAGCCTCTTCCATTGGATACAA-3' |
E1 f | from exon 1 to 5 | 5'-CAAGAGCAGCGGCAACAG-3' |
E 5r | of AβH-J-J transcripts | 5'-AATAAAACTTTGGCATCATCCACTCAAAATCTCC-3' |
qUSF1 f | USF1 transcripts | 5'-TGATGATGCAGTTGACACGGA-3' |
qUSF1 r | 5'-CAGTTGTTGATCTTGTCTCGGC-3' | |
GAPDH f | glyceraldehyde-3-phosphate | 5'-AAGGTCGGAGTCAACGGATTT-3' |
GAPDH r | dehydrogenase mRNA | 5'-ACTGTGGTCATGAGTCCTTCCA-3' |
HMBS f | hydroxymethyl-bilane | 5'-GAACATGCCCTGGAGAAGAATGA-3' |
HMBS r | synthase mRNA | 5'-GGTAGCCTGCATGGTCTCTTGTAT-3' |
ACTIN f | actin mRNA | 5'-TGACGGGGTCAACCACACTGTGCCCATCTA-3' |
ACTIN r | 5'-CTAGAAGCATTTGCGGTGGACGATGGAGGG-3' |